Highly efficient genome editing using oocyte-specific zcas9 transgenic zebrafish

a.
School of Life Science, Shandong University, Jinan 250100, China
b.
Taishan College, Shandong University, Jinan 250100, China
c.
Sorbonne Universités, UPMC Univ Paris 06, CNRS UMR7622, IBPS-Developmental Biology Laboratory, 75005 Paris, France

More Information

Corresponding author: E-mail address: shaoming@sdu.edu.cn (Ming Shao)

摘要

HTML全文

参考文献(19)

[1]	Burger, A., Lindsay, H., Felker, A. et al. Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes Development, 143 (2016),pp. 2025-2037
[2]	Ciruna, B., Weidinger, G., Knaut, H. et al. Production of maternal-zygotic mutant zebrafish by germ-line replacement Proc. Natl. Acad. Sci. U. S. A., 99 (2002),pp. 14919-14924
[3]	Cong, L., Ran, F.A., Cox, D. et al. Multiplex genome engineering using CRISPR/Cas systems Science, 339 (2013),pp. 819-823
[4]	Etard, C., Joshi, S., Stegmaier, J. et al. Tracking of indels by DEcomposition is a simple and effective method to assess efficiency of guide RNAs in zebrafish Zebrafish, 14 (2017),pp. 586-588
[5]	Guo, X., Zhang, T., Hu, Z. et al. Development, 141 (2014),pp. 707-714
[6]	Hwang, W.Y., Fu, Y.F., Reyon, D. et al. Efficient genome editing in zebrafish using a CRISPR-Cas system Nat. Biotechnol., 31 (2013),pp. 227-229
[7]	Kawakami, K., Shima, A., Kawakami, N. Identification of a functional transposase of the Tol2 element, an Ac-like element from the Japanese medaka fish, and its transposition in the zebrafish germ lineage Proc. Natl. Acad. Sci. U. S. A., 97 (2000),pp. 11403-11408
[8]	Liu, D., Wang, Z., Xiao, A. et al. Efficient gene targeting in zebrafish mediated by a zebrafish-codon optimized cas9 and evaluation of off-targeting effect J. Genet. Genomics, 41 (2014),pp. 43-46
[9]	Liu, J.L. Sparks of the CRISPR explosion: applications in medicine and agriculture J. Genet. Genomics, 44 (2017),pp. 413-414
[10]	Mali, P., Yang, L., Esvelt, K.M. et al. RNA-guided human genome engineering via Cas9 Science, 339 (2013),pp. 823-826
[11]	Moreno-Mateos, M.A., Vejnar, C.E., Beaudoin, J.D. et al. Nat. Methods, 10 (2015),pp. 982-988
[12]	Onichtchouk, D., Aduroja, K., Belting, H.G. et al. Transgene driving GFP expression from the promoter of the zona pellucida gene zpc is expressed in oocytes and provides an early marker for gonad differentiation in zebrafish Dev. Dynam., 228 (2003),pp. 393-404
[13]	Shao, M., Wang, M., Liu, Y.Y. et al. Vegetally localised Vrtn functions as a novel repressor to modulate bmp2b transcription during dorsoventral patterning in zebrafish Development, 144 (2017),pp. 3361-3374
[14]	Shao, M., Cheng, X.N., Liu, Y.Y. et al. Transplantation of zebrafish cells by conventional pneumatic microinjector Zebrafish, 15 (2018),pp. 73-76
[15]	Shepherd, I.T., Pietsch, J., Elworthy, S. et al. Roles for GFRα1 receptors in zebrafish enteric nervous system development Development, 131 (2004),pp. 241-249
[16]	Urasaki, A., Morvan, G., Kawakami, K. Genetics, 174 (2006),pp. 639-649
[17]	Wang, H., Yang, H., Shivalila, C.S. et al. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering Cell, 153 (2013),pp. 910-918
[18]	White, R.J., Collins, J.E., Sealy, I.M. et al. A high-resolution mRNA expression time course of embryonic development in zebrafish eLife, 6 (2017)
[19]	Yin, L., Maddison, L.A., Li, M. et al. Multiplex conditional mutagenesis using transgenic expression of Cas9 and sgRNAs Genetics, 200 (2015),pp. 431-441