Defining gene networks controlling the maintenance and function of the differentiation niche by an in vivo systematic RNAi screen

Yuan Gao^{a, #},
Ying Mao^{a, #},
Rong-Gang Xu^{a, b},
Ruibao Zhu^{a, b},
Ming Zhang^a,
Jin Sun^{a, b},
Da Shen^{a, b},
Ping Peng^{a, b},
Ting Xie^{c
, ,},
Jian-Quan Ni^{b, d
, ,}

a.
PKU-THU Joint Center for Life Sciences, College of Life Sciences, School of Medicine, Tsinghua University, Beijing, 100084, China
b.
Gene Regulatory Lab, School of Medicine, Tsinghua University, Beijing, 100084, China
c.
Stowers Institute for Medical Research, 1000 East 50th Street, Kansas City, MO, 64110, USA
d.
Tsingdao Advanced Research Institute, Tongji University, Qingdao, 266000, China

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Corresponding author: E-mail address: tgx@stowers.org (Ting Xie); E-mail address: nijq@mail.tsinghua.edu.cn (Jian-Quan Ni)

These authors contributed equally.

摘要

- /
Abstract: In the Drosophila ovary, escort cells (ECs) extrinsically control germline stem cell (GSC) maintenance and progeny differentiation. However, the underlying mechanisms remain poorly understood. In this study, we identified 173 EC genes for their roles in controlling GSC maintenance and progeny differentiation by using an in vivo systematic RNAi approach. Of the identified genes, 10 and 163 are required in ECs to promote GSC maintenance and progeny differentiation, respectively. The genes required for progeny differentiation fall into different functional categories, including transcription, mRNA splicing, protein degradation, signal transduction and cytoskeleton regulation. In addition, the GSC progeny differentiation defects caused by defective ECs are often associated with BMP signaling elevation, indicating that preventing BMP signaling is a general functional feature of the differentiation niche. Lastly, exon junction complex (EJC) components, which are essential for mRNA splicing, are required in ECs to promote GSC progeny differentiation by maintaining ECs and preventing BMP signaling. Therefore, this study has identified the major regulators of the differentiation niche, which provides important insights into how stem cell progeny differentiation is extrinsically controlled.
- Germline stem cells /
- Escort cells
These authors contributed equally.
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doi: 10.1016/j.jgg.2018.10.008

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Defining gene networks controlling the maintenance and function of the differentiation niche by an in vivo systematic RNAi screen

Corresponding author: E-mail address: tgx@stowers.org (Ting Xie); E-mail address: nijq@mail.tsinghua.edu.cn (Jian-Quan Ni)

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doi: 10.1016/j.jgg.2018.10.008

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Defining gene networks controlling the maintenance and function of the differentiation niche by an in vivo systematic RNAi screen

Corresponding author: E-mail address: tgx@stowers.org (Ting Xie); E-mail address: nijq@mail.tsinghua.edu.cn (Jian-Quan Ni)

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