Arabidopsis AGC protein kinases IREH1 and IRE3 control root skewing

Xiaozhen Yue^{a, b},
Zhiai Guo^a,
Teng Shi^{a, b},
Lizhen Song^a,
Youfa Cheng^{a, b
, ,}

a.
Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
b.
University of Chinese Academy of Sciences, Beijing 100049, China

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Corresponding author: E-mail address: yfcheng@ibcas.ac.cn (Youfa Cheng)

摘要

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Abstract: AGC protein kinases play important roles in plant growth and development. Several AGC kinases in Arabidopsis have been functionally characterized. However, the “AGC Other” subfamily, including IRE, IREH1, IRE3 and IRE4, has not been well understood. Here, we reported that ireh1 mutants displayed a root skewing phenotype, which can be enhanced by ire3 mutation. IREH1 and IRE3 were expressed in roots, consistent with their function in controlling root skewing. The fluorescence intensities of the microtubule marker KNpro:EGFP-MBD were decreased in ireh1, ire3 and ireh1 ire3 mutants compared to wild type. The microtubule arrangements in ireh1 and ireh1 ire3 mutants were also altered. IREH1 physically interacted with IRE3 in vitro and in planta. Thus, our findings demonstrate that IREH1 and IRE3 protein kinases play important roles in controlling root skewing, and maintaining microtubule network in Arabidopsis.
- AGC kinases /
- IREH1 /
- IRE3 /
- Root skewing /
- Microtubule

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参考文献(40)

[1]	Barbosa, I.C.R., Hammes, U.Z., Schwechheimer, C. Activation and polarity control of PIN-FORMED auxin transporters by phosphorylation Trends Plant Sci., 23 (2018),pp. 523-538
[2]	Baskin, T.I., Wilson, J.E., Cork, A. et al. Plant Cell Physiol., 35 (1994),pp. 935-942
[3]	Bisgrove, S.R., Lee, Y.R., Liu, B. et al. Plant Cell, 20 (2008),pp. 396-410
[4]	Bögre, L. Trends Plant Sci., 8 (2003),pp. 424-431
[5]	Briggs, W.R., Christie, J.M. Phototropins 1 and 2: versatile plant blue-light receptors Trends Plant Sci., 7 (2002),pp. 204-210
[6]	Cheng, Y., Dai, X., Zhao, Y. Genes Dev., 20 (2006),pp. 1790-1799
[7]	Cheng, Y., Qin, G., Dai, X. et al. Proc. Natl. Acad. Sci. U. S. A., 105 (2008),pp. 21017-21022
[8]	Chudinova, E.M., Karpov, P.A., Fokin, A.I. et al. Planta, 246 (2017),pp. 959-969
[9]	Christensen, S.K., Dagenais, N., Chory, J. et al. Regulation of auxin response by the protein kinase PINOID Cell, 100 (2000),pp. 469-478
[10]	Clough, S.J., Bent, A.F. Plant J., 16 (1998),pp. 735-743
[11]	Cui, X., Guo, Z., Song, L. et al. PLoS Genet., 12 (2016),p. e1005923
[12]	de la Fuente van Bentem, S., Anrather, D., Dohnal, I. et al. J. Proteome Res., 7 (2008),pp. 2458-2470
[13]	Enugutti, B., Kirchhelle, C., Oelschner, M. et al. Proc. Natl. Acad. Sci. U. S. A., 109 (2012),pp. 15060-15065
[14]	Furutani, I., Watanabe, Y., Prieto, R. et al. Development, 127 (2000),pp. 4443-4453
[15]	Gibson, D.G., Young, L., Chuang, R.Y. et al. Enzymatic assembly of DNA molecules up to several hundred kilobases Nat. Methods, 6 (2009),pp. 343-345
[16]	Haga, K., Frank, L., Kimura, T. et al. Plant Cell Physiol., 59 (2018),pp. 1060-1071
[17]	Hain, D., Langlands, A., Sonnenberg, H.C. et al. Development, 141 (2014),pp. 2119-2130
[18]	Hasenstein, K.H., Blancaflor, E.B., Lee, J.S. The microtubule cytoskeleton does not integrate auxin transport and gravitropism in maize roots Plant Physiol., 105 (1999),pp. 729-738
[19]	Hajdukiewicz, P., Svab, Z., Maliga, P. Plant Mol. Biol., 25 (1994),pp. 989-994
[20]	Karpov, P.A., Nadezhdina, E.S., Yemets, A.I. et al. Bioinformatic search of plant microtubule- and cell cycle related serine-threonine protein kinases BMC Genomics, 11 (2010),p. S14
[21]	Marhava, P., Bassukas, A.E.L., Zourelidou, M. et al. A molecular rheostat adjusts auxin flux to promote root protophloem differentiation Nature, 558 (2018),pp. 297-300
[22]	Morejohn, L.C.
[23]	Naoi, K., Hashimoto, T. Plant Cell, 16 (2004),pp. 1841-1853
[24]	Nemoto, K., Seto, T., Takahashi, H. et al. Phytochemistry, 72 (2011),pp. 1136-1144
[25]	Nakajima, K., Furutani, I., Tachimoto, H. et al. Plant Cell, 16 (2004),pp. 1178-1190
[26]	Oyama, T., Shimura, Y., Okada, K. Plant J., 30 (2002),pp. 289-299
[27]	Pearce, L.R., Komander, D., Alessi, D.R. The nuts and bolts of AGC protein kinases Nat. Rev. Mol. Cell Biol., 11 (2010),pp. 9-22
[28]	Perrin, R.M., Wang, Y., Yuen, C.Y.L. et al. Plant J., 49 (2007),pp. 961-971
[29]	Qi, B., Zheng, H. Plant J., 76 (2013),pp. 380-392
[30]	Roy, R., Bassham, D.C. Root growth movements: waving and skewing Plant Sci., 221–222 (2014),pp. 42-47
[31]	Roy, R., Bassham, D.C. BMC Plant Biol., 17 (2017),p. 73
[32]	Sedbrook, J., Ehrhardt, D., Fisher, S. et al. Plant Cell, 16 (2004),pp. 1506-1520
[33]	Shoji, T., Narita, N.N., Hayashi, K. et al. Plant Physiol., 136 (2004),pp. 3933-3944
[34]	Thitamadee, S., Tuchihara, K., Hashimoto, T. Nature, 417 (2002),pp. 193-196
[35]	Valiente, M., Andres-Pons, A., Gomar, B. et al. Binding of PTEN to specific PDZ domains contributes to PTEN protein stability and phosphorylation by microtubule-associated serine/threonine kinases J. Biol. Chem., 280 (2005),pp. 28936-28943
[36]	Vaughn, L.M., Masson, P.H. G3, 1 (2011),pp. 105-115
[37]	Wang, Q., Huang, S. Mol. Plant, 7 (2014),pp. 1397-1401
[38]	Willige, B.C., Ahlers, S., Zourelidou, M. et al. Plant Cell, 25 (2013),pp. 1674-1688
[39]	Whittington, A.T., Vugrek, O., Wei, K.J. et al. MOR1 is essential for organizing cortical microtubules in plants Nature, 411 (2001),pp. 610-613
[40]	Zourelidou, M., Muller, I., Willige, B.C. et al. Development, 136 (2009),pp. 627-636