An effective strategy to establish a male sterility mutant mini-library by CRISPR/Cas9-mediated knockout of anther-specific genes in rice

Kun Ma^{1, 2, 3, 4, #},
Jingluan Han^{1, 2, 3, 4, #},
Yu Hao^{5, 6},
Zhongfang Yang^{5, 6},
Junyu Chen⁷,
Yao-Guang Liu^{8, 9, 10},
Qinlong Zhu^{11, 12
, ,},
Letian Chen^{13, 14, 15, 16
, ,}

1.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China
2.
Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, South China Agricultural University, Guangzhou, 510642, China
3.
Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, South China Agricultural University, Guangzhou, 510642, China
4.
College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China
5.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China
6.
College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China
7.
College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China
8.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China
9.
Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, South China Agricultural University, Guangzhou, 510642, China
10.
College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China
11.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China
12.
College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China
13.
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China
14.
Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, South China Agricultural University, Guangzhou, 510642, China
15.
Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, South China Agricultural University, Guangzhou, 510642, China
16.
College of Life Sciences, South China Agricultural University, Guangzhou, 510642, China

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Corresponding author: E-mail address: zhuql@scau.edu.cn (Qinlong Zhu); E-mail address: lotichen@scau.edu.cn (Letian Chen)

These authors contributed equally to this work.

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These authors contributed equally to this work.

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参考文献(17)

[1]	Chen, L., Liu, Y.G. Male sterility and fertility restoration in crops Annu. Rev. Plant Biol., 65 (2014),pp. 579-606
[2]	Chern, C.G., Fan, M.J., Yu, S.M. et al. A rice phenomics study-phenotype scoring and seed propagation of a T-DNA insertion-induced rice mutant population Plant Mol. Biol., 65 (2007),pp. 427-438
[3]	Deveshwar, P., Bovill, W.D., Sharma, R. et al. Analysis of anther transcriptomes to identify genes contributing to meiosis and male gametophyte development in rice BMC Plant Biol., 11 (2011),p. 78
[4]	Gasiunas, G., Barrangou, R., Horvath, P. et al. Cas9-crRNA ribonucleoprotein complex mediates specific DNA cleavage for adaptive immunity in bacteria Proc. Natl. Acad. Sci. U. S. A., 109 (2012),pp. E2579-E2586
[5]	Liu, W., Xie, X., Ma, X. et al. DSDecode: a web-based tool for decoding of sequencing chromatograms for genotyping of targeted mutations Mol. Plant, 8 (2015),pp. 1431-1433
[6]	Lu, Y., Ye, X., Guo, R. et al. Genome-wide targeted mutagenesis in rice using the CRISPR/Cas9 system Mol. Plant, 10 (2017),pp. 1242-1245
[7]	Ma, X., Zhang, Q., Zhu, Q. et al. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants Mol. Plant, 8 (2015),pp. 1274-1284
[8]	Ma, X., Chen, L., Zhu, Q. et al. Rapid decoding of sequence-specific nuclease-induced heterozygous and biallelic mutations by direct sequencing of PCR products Mol. Plant, 8 (2015),pp. 1285-1287
[9]	Meng, X., Yu, H., Zhang, Y. et al. Construction of a genome-wide mutant library in rice using CRISPR/Cas9 Mol. Plant (2017),pp. 1238-1241
[10]	Moscou, M.J., Bogdanove, A.J. A simple cipher governs DNA recognition by TAL effectors Science, 326 (2009),p. 1501
[11]	Raitskin, O., Patron, N.J. Multi-gene engineering in plants with RNA-guided Cas9 nuclease Curr. Opin. Biotechnol., 37 (2016),pp. 69-75
[12]	Smith, J., Bibikova, M., Whitby, F.G. et al. Requirements for double-strand cleavage by chimeric restriction enzymes with zinc finger DNA-recognition domains Nucleic Acids Res., 28 (2000),pp. 3361-3369
[13]	Shi, J., Cui, M., Yang, L. et al. Genetic and biochemical mechanisms of pollen wall development Trends Plant Sci., 20 (2015),pp. 741-753
[14]	Wei, F.J., Droc, G., Guiderdoni, E. et al. International consortium of rice mutagenesis: resources and beyond Rice, 6 (2013),p. 39
[15]	Xie, X., Ma, X., Zhu, Q. et al. CRISPR-GE: a convenient software toolkit for CRISPR-based genome editing Mol. Plant, 10 (2017),pp. 1246-1249
[16]	Yang, X., Liang, W., Chen, M. et al. Rice fatty acyl-CoA synthetase OsACOS12 is required for tapetum programmed cell death and male fertility Planta, 246 (2017),pp. 105-122
[17]	Zhang, F., Wen, Y., Guo, X. CRISPR/Cas9 for genome editing: progress, implications and challenges Hum. Mol. Genet., 23 (2014),pp. R40-R46

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doi: 10.1016/j.jgg.2019.03.005

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An effective strategy to establish a male sterility mutant mini-library by CRISPR/Cas9-mediated knockout of anther-specific genes in rice

Corresponding author: E-mail address: zhuql@scau.edu.cn (Qinlong Zhu); E-mail address: lotichen@scau.edu.cn (Letian Chen)

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doi: 10.1016/j.jgg.2019.03.005

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An effective strategy to establish a male sterility mutant mini-library by CRISPR/Cas9-mediated knockout of anther-specific genes in rice

Corresponding author: E-mail address: zhuql@scau.edu.cn (Qinlong Zhu); E-mail address: lotichen@scau.edu.cn (Letian Chen)

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