RSBP15 interacts with and stabilizes dRSPH3 during sperm axoneme assembly in Drosophila

Ya Wang^{a, b},
Rui Xu^{c, d},
Yiwei Cheng^{a, b},
Haowei Cao^c,
Zibin Wang^a,
Tianyu Zhu^{a, b},
Jiayin Jiang^{a, b},
Hao Zhang^{a, b},
Chang Wang^{a, b},
Lin Qi^{a, b},
Mingxi Liu^{a, b},
Xuejiang Guo^{a, b
, ,},
Juan Huang^{c, d
, ,},
Jiahao Sha^{a, b
, ,}

a.
State Key Laboratory of Reproductive Medicine, Nanjing Medical University, Nanjing, 211166, China
b.
Department of Histology and Embryology, Nanjing Medical University, Nanjing, 211166, China
c.
Department of Medical Genetics, Nanjing Medical University, Nanjing, 211166, China
d.
Jiangsu Key Laboratory of Xenotransplantation, Nanjing Medical University, Nanjing, 211166, China

More Information

Corresponding author: E-mail address: guo_xuejiang@njmu.edu.cn (Xuejiang Guo); E-mail address: huangjuan@njmu.edu.cn (Juan Huang); E-mail address: shajh@njmu.edu.cn (Jiahao Sha)

摘要

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Abstract: Flagellum in sperm is composed of over 200 different proteins and is essential for sperm motility. In particular, defects in the assembly of the radial spoke in the flagellum result in male infertility due to loss of sperm motility. However, mechanisms regulating radial spoke assembly remain unclear in metazoans. Here, we identified a novel Drosophila protein radial spoke binding protein 15 (RSBP15) which plays an important role in regulating radial spoke assembly. Loss of RSBP15 results in complete lack of mature sperms in seminal vesicles (SVs), asynchronous individualization complex (IC) and defective “9 + 2” structure in flagella. RSBP15 is colocalized with dRSPH3 in sperm flagella, and interacts with dRSPH3 through its DD_R_PKA superfamily domain which is important for the stabilization of dRSPH3. Moreover, loss of dRSPH3, as well as dRSPH1, dRSPH4a and dRSPH9, showed similar phenotypes to rsbp15 mutant. Together, our results suggest that RSBP15 acts in stabilizing the radial spoke protein complex to anchor and strengthen the radial spoke structures in sperm flagella.
- RSBP15 /
- RSPH3 /
- Flagellum /
- Radial spoke

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参考文献(43)

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doi: 10.1016/j.jgg.2019.05.001

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RSBP15 interacts with and stabilizes dRSPH3 during sperm axoneme assembly in Drosophila

Corresponding author: E-mail address: guo_xuejiang@njmu.edu.cn (Xuejiang Guo); E-mail address: huangjuan@njmu.edu.cn (Juan Huang); E-mail address: shajh@njmu.edu.cn (Jiahao Sha)

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doi: 10.1016/j.jgg.2019.05.001

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RSBP15 interacts with and stabilizes dRSPH3 during sperm axoneme assembly in Drosophila

Corresponding author: E-mail address: guo_xuejiang@njmu.edu.cn (Xuejiang Guo); E-mail address: huangjuan@njmu.edu.cn (Juan Huang); E-mail address: shajh@njmu.edu.cn (Jiahao Sha)

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