Site-directed RNA editing (SDRE): Off-target effects and their countermeasures

Shaoshuai Mao^{a, b, c},
Yajing Liu^{a, b, c},
Shisheng Huang^{a, b, c},
Xingxu Huang^a,
Tian Chi^{a, d
, ,}

a.
School of Life Sciences and Technology, ShanghaiTech University, Shanghai, 201210, China
b.
CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China
c.
University of Chinese Academy of Sciences, Beijing, 100049, China
d.
Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA

More Information

Corresponding author: E-mail address: chitian@shanghaitech.edu.cn (Tian Chi)

摘要

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Abstract: Site-directed RNA editing (SDRE) is invaluable to basic research and clinical applications and has emerged as a new frontier in genome editing. The past few years have witnessed a surge of interest in SDRE, with SDRE tools emerging at a breathtaking pace. However, off-target effects of SDRE remain a tough problem, which constitutes a major hurdle to their clinical applications. Here we discuss the diverse strategies for combating off-target editing, drawing lessons from the published studies as well as our ongoing research. Overall, SDRE is still at its infancy, with significant challenges and exciting opportunities ahead.
- Gene editing /
- RNA editing /
- Cas13 /
- Off-target /
- Specificity

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