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The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice

Xiaowei Fan,  Pengfei Wang,  Feixiang Qi,  Yong Hu,  Shuangle Li,  Jia Zhang,  Liwen Liang,  Zhanyi Zhang,  Juhong Liu,  Lizhong Xiong,  Yonghzong Xing

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文章导航 > Journal of Genetics and Genomics  > 2023 > 优先出版
Xiaowei Fan, Pengfei Wang, Feixiang Qi, Yong Hu, Shuangle Li, Jia Zhang, Liwen Liang, Zhanyi Zhang, Juhong Liu, Lizhong Xiong, Yonghzong Xing. The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice[J]. 遗传学报. doi: 10.1016/j.jgg.2023.03.002
引用本文: Xiaowei Fan, Pengfei Wang, Feixiang Qi, Yong Hu, Shuangle Li, Jia Zhang, Liwen Liang, Zhanyi Zhang, Juhong Liu, Lizhong Xiong, Yonghzong Xing. The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice[J]. 遗传学报. doi: 10.1016/j.jgg.2023.03.002
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Xiaowei Fan, Pengfei Wang, Feixiang Qi, Yong Hu, Shuangle Li, Jia Zhang, Liwen Liang, Zhanyi Zhang, Juhong Liu, Lizhong Xiong, Yonghzong Xing. The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice[J]. Journal of Genetics and Genomics. doi: 10.1016/j.jgg.2023.03.002
Citation: Xiaowei Fan, Pengfei Wang, Feixiang Qi, Yong Hu, Shuangle Li, Jia Zhang, Liwen Liang, Zhanyi Zhang, Juhong Liu, Lizhong Xiong, Yonghzong Xing. The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice[J]. Journal of Genetics and Genomics. doi: 10.1016/j.jgg.2023.03.002
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The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice

doi: 10.1016/j.jgg.2023.03.002

  • 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, China;

  • 2 Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China

基金项目: 

This study was supported by the Natural Science Foundation of China (U20A2031) and the Earmarked Fund for China Agriculture Research System (CARS-01).

详细信息
    通讯作者:

    Yonghzong Xing,Email address:yzxing@mail.hzau.edu.cn

The CCT transcriptional activator Ghd2 constantly delays the heading date by upregulating CO3 in rice

  • 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei 430070, China;

  • 2 Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China

Funds: 

This study was supported by the Natural Science Foundation of China (U20A2031) and the Earmarked Fund for China Agriculture Research System (CARS-01).

    摘要
  • 摘要: CONSTANS, CO-like and TOC1 (CCT) family genes play important roles in regulating heading date, which exerts a large impact on the regional and seasonal adaptation of rice. Previous studies have shown that Grain number, plant height, and heading date2 (Ghd2) exhibits a negative response to drought stress by directly upregulating Rubisco activase and exerting a negative effect on heading date. However, the target gene of Ghd2 regulating heading date is still unknown. In this study, CO3 is identified by analyzing ChIP-seq data. Ghd2 activates CO3 expression by binding to the CO3 promoter through its CCT domain. EMSA experiments show that the motif CCACTA in the CO3 promoter was recognized by Ghd2. A comparison of the heading dates among plants with CO3 knocked out or overexpressed and double mutants overexpressing Ghd2 with CO3 knocked out shows that CO3 negatively and constantly regulates flowering by repressing the transcription of Ehd1, Hd3a and RFT1. In addition, the target genes of CO3 are explored via a comprehensive analysis of DAP-seq data and RNA-seq data. Taken together, these results suggest that Ghd2 directly binds to the downstream gene CO3, and the Ghd2-CO3 module constantly delays heading date via the Ehd1 mediated pathway.
    Abstract: CONSTANS, CO-like and TOC1 (CCT) family genes play important roles in regulating heading date, which exerts a large impact on the regional and seasonal adaptation of rice. Previous studies have shown that Grain number, plant height, and heading date2 (Ghd2) exhibits a negative response to drought stress by directly upregulating Rubisco activase and exerting a negative effect on heading date. However, the target gene of Ghd2 regulating heading date is still unknown. In this study, CO3 is identified by analyzing ChIP-seq data. Ghd2 activates CO3 expression by binding to the CO3 promoter through its CCT domain. EMSA experiments show that the motif CCACTA in the CO3 promoter was recognized by Ghd2. A comparison of the heading dates among plants with CO3 knocked out or overexpressed and double mutants overexpressing Ghd2 with CO3 knocked out shows that CO3 negatively and constantly regulates flowering by repressing the transcription of Ehd1, Hd3a and RFT1. In addition, the target genes of CO3 are explored via a comprehensive analysis of DAP-seq data and RNA-seq data. Taken together, these results suggest that Ghd2 directly binds to the downstream gene CO3, and the Ghd2-CO3 module constantly delays heading date via the Ehd1 mediated pathway.
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  • 收稿日期:  2023-02-27
  • 录用日期:  2023-03-03
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