LSM14B coordinates protein component expression in the P-body and controls oocyte maturation

Huiru Zhang; Tao Zhang; Xiang Wan; Chang Chen; Shu Wang; Dongdong Qin; Lufan Li; Luping Yu; Xin Wu

doi:10.1016/j.jgg.2023.07.006

LSM14B coordinates protein component expression in the P-body and controls oocyte maturation

doi: 10.1016/j.jgg.2023.07.006

a State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
b Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, Jiangsu 210029, China

基金项目:

We are grateful to Dr. Henyu Fan at Zhejiang University for his valuable comments and review of this manuscript. We thank Nature Research Editing Service and American Journal Experts for help with reviewing the manuscript for grammar. We also thank all the members of the Wu Laboratory for their valuable assistance. The work was supported by the National Key R&D Program of China (2021YFC2700201 to XW) and the National Natural Science Foundation of China (32070831, 32270897 to XW).

详细信息

通讯作者:
Luping Yu,E-mail:yuluping@njmu.edu.cn

Xin Wu,E-mail:xinwu@njmu.edu.cn

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- 文章访问数: 142
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出版历程
- 收稿日期: 2023-03-23
- 录用日期: 2023-07-14
- 修回日期: 2023-07-13
- 网络出版日期: 2023-07-21

LSM14B coordinates protein component expression in the P-body and controls oocyte maturation

a State Key Laboratory of Reproductive Medicine and Offspring Health, Nanjing Medical University, Nanjing, Jiangsu 210029, China;
b Center for Reproductive Medicine and Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, Jiangsu 210029, China

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摘要

摘要: The generation of mature and healthy oocytes is the most critical event in the entire female reproductive process, and the mechanisms regulating this process remain to be studied. Here, we demonstrate that LSM family member 14B (LSM14B) regulates oocyte maturation, and the loss of LSM14B in mouse ovaries leads to abnormal oocyte MII arrest and female infertility. Next, we find the aberrant transcriptional activation, indicated by abnormal non-surrounded nucleolus (NSN) and surrounded nucleolus (SN) oocyte proportions, and abnormal chromosome assembly and segregation in Lsm14b-deficient mouse oocytes. The global transcriptome analysis suggests that many transcripts involved in cytoplasmic processing body (P-body) function are altered in Lsm14b-deficient mouse oocytes. Deletion of Lsm14b results in the expression and/or localization changes of P-body components (such as LSM14A, DCP1A, and 4E-T). Notably, DDX6, a key component of the P-body, is downregulated and accumulates in the nuclei in Lsm14b-deficient mouse oocytes. Taken together, our data suggest that LSM14B links mouse oocyte maturation to female fertility through regulation of the P-body.
- LSM14B /
- P-body /
- DDX6 /
- MII arrest /
- Chromatin configuration
Abstract: The generation of mature and healthy oocytes is the most critical event in the entire female reproductive process, and the mechanisms regulating this process remain to be studied. Here, we demonstrate that LSM family member 14B (LSM14B) regulates oocyte maturation, and the loss of LSM14B in mouse ovaries leads to abnormal oocyte MII arrest and female infertility. Next, we find the aberrant transcriptional activation, indicated by abnormal non-surrounded nucleolus (NSN) and surrounded nucleolus (SN) oocyte proportions, and abnormal chromosome assembly and segregation in Lsm14b-deficient mouse oocytes. The global transcriptome analysis suggests that many transcripts involved in cytoplasmic processing body (P-body) function are altered in Lsm14b-deficient mouse oocytes. Deletion of Lsm14b results in the expression and/or localization changes of P-body components (such as LSM14A, DCP1A, and 4E-T). Notably, DDX6, a key component of the P-body, is downregulated and accumulates in the nuclei in Lsm14b-deficient mouse oocytes. Taken together, our data suggest that LSM14B links mouse oocyte maturation to female fertility through regulation of the P-body.
- LSM14B /
- P-body /
- DDX6 /
- MII arrest /
- Chromatin configuration

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参考文献(62)

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LSM14B coordinates protein component expression in the P-body and controls oocyte maturation

doi: 10.1016/j.jgg.2023.07.006

通讯作者:
Luping Yu,E-mail:yuluping@njmu.edu.cn

Xin Wu,E-mail:xinwu@njmu.edu.cn

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LSM14B coordinates protein component expression in the P-body and controls oocyte maturation

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留言板

LSM14B coordinates protein component expression in the P-body and controls oocyte maturation

doi: 10.1016/j.jgg.2023.07.006

通讯作者: Luping Yu,E-mail:yuluping@njmu.edu.cn Xin Wu,E-mail:xinwu@njmu.edu.cn

计量

出版历程

LSM14B coordinates protein component expression in the P-body and controls oocyte maturation

计量

出版历程

目录

通讯作者:
Luping Yu,E-mail:yuluping@njmu.edu.cn

Xin Wu,E-mail:xinwu@njmu.edu.cn