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Phosphorylation of KAT-2B by WKS1/Yr36 redirects the lipid flux to jasmonates to enhance resistance against wheat stripe rust

Yan Yan,  Xiao-Ming Li,  Yun Chen,  Tian-Tian Wu,  Ci-Hang Ding,  Mei-Qi Zhang,  YueTing Guo,  Chu-Yang Wang,  Junli Zhang,  Xuebin Zhang,  Awais Rasheed,  Shengchun Xu,  Meng-Lu Wang,  Zhongfu Ni,  Qixin Sun,  Jin-Ying Gou

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文章导航 > Journal of Genetics and Genomics  > 2023 > 优先出版
Yan Yan, Xiao-Ming Li, Yun Chen, Tian-Tian Wu, Ci-Hang Ding, Mei-Qi Zhang, YueTing Guo, Chu-Yang Wang, Junli Zhang, Xuebin Zhang, Awais Rasheed, Shengchun Xu, Meng-Lu Wang, Zhongfu Ni, Qixin Sun, Jin-Ying Gou. Phosphorylation of KAT-2B by WKS1/Yr36 redirects the lipid flux to jasmonates to enhance resistance against wheat stripe rust[J]. 遗传学报. doi: 10.1016/j.jgg.2023.08.009
引用本文: Yan Yan, Xiao-Ming Li, Yun Chen, Tian-Tian Wu, Ci-Hang Ding, Mei-Qi Zhang, YueTing Guo, Chu-Yang Wang, Junli Zhang, Xuebin Zhang, Awais Rasheed, Shengchun Xu, Meng-Lu Wang, Zhongfu Ni, Qixin Sun, Jin-Ying Gou. Phosphorylation of KAT-2B by WKS1/Yr36 redirects the lipid flux to jasmonates to enhance resistance against wheat stripe rust[J]. 遗传学报. doi: 10.1016/j.jgg.2023.08.009
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Yan Yan, Xiao-Ming Li, Yun Chen, Tian-Tian Wu, Ci-Hang Ding, Mei-Qi Zhang, YueTing Guo, Chu-Yang Wang, Junli Zhang, Xuebin Zhang, Awais Rasheed, Shengchun Xu, Meng-Lu Wang, Zhongfu Ni, Qixin Sun, Jin-Ying Gou. Phosphorylation of KAT-2B by WKS1/Yr36 redirects the lipid flux to jasmonates to enhance resistance against wheat stripe rust[J]. Journal of Genetics and Genomics. doi: 10.1016/j.jgg.2023.08.009
Citation: Yan Yan, Xiao-Ming Li, Yun Chen, Tian-Tian Wu, Ci-Hang Ding, Mei-Qi Zhang, YueTing Guo, Chu-Yang Wang, Junli Zhang, Xuebin Zhang, Awais Rasheed, Shengchun Xu, Meng-Lu Wang, Zhongfu Ni, Qixin Sun, Jin-Ying Gou. Phosphorylation of KAT-2B by WKS1/Yr36 redirects the lipid flux to jasmonates to enhance resistance against wheat stripe rust[J]. Journal of Genetics and Genomics. doi: 10.1016/j.jgg.2023.08.009
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Phosphorylation of KAT-2B by WKS1/Yr36 redirects the lipid flux to jasmonates to enhance resistance against wheat stripe rust

doi: 10.1016/j.jgg.2023.08.009

  • a Frontiers Science Center for Molecular Design Breeding(MOE), China Agricultural University, Beijing 100193, China;

  • b Xianghu Laboratory, Hangzhou 311231, China;

  • c School of Life Sciences, Fudan University, Shanghai 200438, China;

  • d Institute of Metabolism and Integrative Biology, Fudan University, Shanghai 200438, China;

  • e State Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng, Henan 475004, China;

  • f International Maize and Wheat Improvement Center(CIMMYT), c/o Chinese Academy of Agricultural Sciences, Beijing 100081, China

基金项目: 

We appreciate Prof. Jorge Dubcovsky at UC Davis for sharing the ProUbi::TAP-WKS1.1 and ProNP::WKS1 transgenic lines. This study was supported by the National Natural Science Foundation of China (32372557 and 31972350), the China Postdoctoral Science Foundation (2021M700850), an open project of the State Key Laboratory of Crop Stress Adaptation and Improvement at Henan University, and the Central Government guided Local Science and Technology Development Funds (2023ZY1016).

详细信息
    通讯作者:

    Jin-Ying Gou, jygou@cau.edu.cn

Phosphorylation of KAT-2B by WKS1/Yr36 redirects the lipid flux to jasmonates to enhance resistance against wheat stripe rust

  • a Frontiers Science Center for Molecular Design Breeding(MOE), China Agricultural University, Beijing 100193, China;

  • b Xianghu Laboratory, Hangzhou 311231, China;

  • c School of Life Sciences, Fudan University, Shanghai 200438, China;

  • d Institute of Metabolism and Integrative Biology, Fudan University, Shanghai 200438, China;

  • e State Key Laboratory of Crop Stress Adaptation and Improvement, Henan Joint International Laboratory for Crop Multi-Omics Research, School of Life Sciences, Henan University, Kaifeng, Henan 475004, China;

  • f International Maize and Wheat Improvement Center(CIMMYT), c/o Chinese Academy of Agricultural Sciences, Beijing 100081, China

Funds: 

We appreciate Prof. Jorge Dubcovsky at UC Davis for sharing the ProUbi::TAP-WKS1.1 and ProNP::WKS1 transgenic lines. This study was supported by the National Natural Science Foundation of China (32372557 and 31972350), the China Postdoctoral Science Foundation (2021M700850), an open project of the State Key Laboratory of Crop Stress Adaptation and Improvement at Henan University, and the Central Government guided Local Science and Technology Development Funds (2023ZY1016).

    摘要
  • 摘要:

    Wheat (Triticum aestivum) is one of the most essential human energy and protein sources. However, wheat production is threatened by devastating fungal diseases, such as stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici(Pst). Here, we reveal that the alternations in chloroplast lipid profiles and the accumulation of jasmonate (JA) in the necrosis region activate JA signaling and trigger the host defense. The collapse of chloroplasts in the necrosis region results in accumulations of polyunsaturated membrane lipids and the lipid-derived phytohormone, JA, in transgenic lines of Yr36 that encodes Wheat Kinase START 1 (WKS1), a high-temperature-dependent adultplant resistance protein. WKS1.1, a protein encoded by a full-length splicing variant of WKS1, phosphorylates and enhances the activity of keto-acyl thiolase (KAT-2B), a critical enzyme catalyzing the β-oxidation reaction in JA biosynthesis. The premature stop mutant, kat-2b, accumulates less JA and shows defects in the host defense against Pst. Conversely, over-expression of KAT-2B results in a higher level of JA and limits the growth of Pst. Moreover, JA inhibits the growth and reduces pustule densities of Pst. This study illustrates the WKS1.1-KAT-2B-JA pathway enhancing wheat defense against fungal pathogens to attenuate yield loss.

    Abstract:

    Wheat (Triticum aestivum) is one of the most essential human energy and protein sources. However, wheat production is threatened by devastating fungal diseases, such as stripe rust, caused by Puccinia striiformis Westend. f. sp. tritici(Pst). Here, we reveal that the alternations in chloroplast lipid profiles and the accumulation of jasmonate (JA) in the necrosis region activate JA signaling and trigger the host defense. The collapse of chloroplasts in the necrosis region results in accumulations of polyunsaturated membrane lipids and the lipid-derived phytohormone, JA, in transgenic lines of Yr36 that encodes Wheat Kinase START 1 (WKS1), a high-temperature-dependent adultplant resistance protein. WKS1.1, a protein encoded by a full-length splicing variant of WKS1, phosphorylates and enhances the activity of keto-acyl thiolase (KAT-2B), a critical enzyme catalyzing the β-oxidation reaction in JA biosynthesis. The premature stop mutant, kat-2b, accumulates less JA and shows defects in the host defense against Pst. Conversely, over-expression of KAT-2B results in a higher level of JA and limits the growth of Pst. Moreover, JA inhibits the growth and reduces pustule densities of Pst. This study illustrates the WKS1.1-KAT-2B-JA pathway enhancing wheat defense against fungal pathogens to attenuate yield loss.

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  • 收稿日期:  2023-08-28
  • 录用日期:  2023-08-29
  • 修回日期:  2023-08-29
  • 网络出版日期:  2023-09-02

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