<i>TaACTIN7-D</i> regulates plant height and grain shape in bread wheat

Xiongtao Li; Beilu Cao; Dejie Du; Long Song; Lulu Tian; Xiaoming Xie; Zhaoyan Chen; Yanpeng Ding; Xuejiao Cheng; Yingyin Yao; Weilong Guo; Zhenqi Su; Qixin Sun; Zhongfu Ni; Lingling Chai; Jie Liu

doi:10.1016/j.jgg.2023.09.001

TaACTIN7-D regulates plant height and grain shape in bread wheat

doi: 10.1016/j.jgg.2023.09.001

a Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, P.R. China;
b State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, P.R. China

基金项目:

We thank Tonglin Mao and Yan Li (China Agricultural University) for valuable suggestions on the study. This research was supported by the grants from National Key Research and Development Program of China (2022YFF1003401 to Jie Liu), Hainan Yazhou Bay Seed Laboratory (B21HJ0111 to Zhongfu Ni) and National Natural Science Foundation of China (31991210 to Qixin Sun and 32072055 to Jie Liu).

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通讯作者:
Lingling Chai,E-mail addresses:chaizz901230@163.com

Jie Liu,E-mail addresses:jieliu@cau.edu.cn

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- 收稿日期: 2023-07-13
- 修回日期: 2023-09-03
- 网络出版日期: 2023-09-13

TaACTIN7-D regulates plant height and grain shape in bread wheat

a Frontiers Science Center for Molecular Design Breeding, China Agricultural University, Beijing, 100193, P.R. China;
b State Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, P.R. China

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摘要

摘要: Exploitation of new gene resources and genetic networks contributing to the control of crop yield-related traits, such as plant height and grain size and shape, may enable us to breed modern high-yielding wheat varieties through molecular methods. In this study, via ethylmethanesulfonate (EMS) mutagenesis, we identified a wheat mutant plant, mu-597, that shows semi-dwarf plant architecture and round grain shape. Through bulked segregant RNA-seq and map-based cloning, the causal gene for the semi-dwarf phenotype of mu-597 was located. We found that a single-base mutation in the coding region of TaACTIN7-D (TaACT7-D), leading to a Gly-to-Ser (G65S) amino acid mutation at the 65^th residue of the deduced TaACT7-D protein, can explain the semi- dwarfism and round grain shape of mu-597. Further evidence shows that the G65S mutation in TaACT7-D hinders the polymerization of actin from monomeric (G-actin) to filamentous (F-actin) status while attenuates wheat responses to multiple phytohormones, including brassinosteroids, auxin, and gibberellin. Together, these findings not only define a new semi-dwarfing gene resource that can be potentially used to design plant height and grain shape of bread wheat but also establish a direct link between actin structure modulation and phytohormone signal transduction.
- wheat (Triticum aestivum L.) /
- plant height /
- grain shape /
- TaACT7-D /
- phytohormone signalling
Abstract: Exploitation of new gene resources and genetic networks contributing to the control of crop yield-related traits, such as plant height and grain size and shape, may enable us to breed modern high-yielding wheat varieties through molecular methods. In this study, via ethylmethanesulfonate (EMS) mutagenesis, we identified a wheat mutant plant, mu-597, that shows semi-dwarf plant architecture and round grain shape. Through bulked segregant RNA-seq and map-based cloning, the causal gene for the semi-dwarf phenotype of mu-597 was located. We found that a single-base mutation in the coding region of TaACTIN7-D (TaACT7-D), leading to a Gly-to-Ser (G65S) amino acid mutation at the 65^th residue of the deduced TaACT7-D protein, can explain the semi- dwarfism and round grain shape of mu-597. Further evidence shows that the G65S mutation in TaACT7-D hinders the polymerization of actin from monomeric (G-actin) to filamentous (F-actin) status while attenuates wheat responses to multiple phytohormones, including brassinosteroids, auxin, and gibberellin. Together, these findings not only define a new semi-dwarfing gene resource that can be potentially used to design plant height and grain shape of bread wheat but also establish a direct link between actin structure modulation and phytohormone signal transduction.
- wheat (Triticum aestivum L.) /
- plant height /
- grain shape /
- TaACT7-D /
- phytohormone signalling

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TaACTIN7-D regulates plant height and grain shape in bread wheat

doi: 10.1016/j.jgg.2023.09.001

通讯作者:
Lingling Chai,E-mail addresses:chaizz901230@163.com

Jie Liu,E-mail addresses:jieliu@cau.edu.cn

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TaACTIN7-D regulates plant height and grain shape in bread wheat

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TaACTIN7-D regulates plant height and grain shape in bread wheat

doi: 10.1016/j.jgg.2023.09.001

通讯作者: Lingling Chai,E-mail addresses:chaizz901230@163.com Jie Liu,E-mail addresses:jieliu@cau.edu.cn

计量

出版历程

TaACTIN7-D regulates plant height and grain shape in bread wheat

计量

出版历程

目录

通讯作者:
Lingling Chai,E-mail addresses:chaizz901230@163.com

Jie Liu,E-mail addresses:jieliu@cau.edu.cn