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Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice

Yaling Wang,  Xueying Huang,  Guoying Sun,  Jingwen Chen,  Bangguo Wu,  Jiahui Luo,  Shuyan Tang,  Peng Dai,  Feng Zhang,  Jinsong Li,  Lingbo Wang

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文章导航 > Journal of Genetics and Genomics  > 2023 > 优先出版
Yaling Wang, Xueying Huang, Guoying Sun, Jingwen Chen, Bangguo Wu, Jiahui Luo, Shuyan Tang, Peng Dai, Feng Zhang, Jinsong Li, Lingbo Wang. Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice[J]. 遗传学报. doi: 10.1016/j.jgg.2023.09.002
引用本文: Yaling Wang, Xueying Huang, Guoying Sun, Jingwen Chen, Bangguo Wu, Jiahui Luo, Shuyan Tang, Peng Dai, Feng Zhang, Jinsong Li, Lingbo Wang. Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice[J]. 遗传学报. doi: 10.1016/j.jgg.2023.09.002
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Yaling Wang, Xueying Huang, Guoying Sun, Jingwen Chen, Bangguo Wu, Jiahui Luo, Shuyan Tang, Peng Dai, Feng Zhang, Jinsong Li, Lingbo Wang. Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice[J]. Journal of Genetics and Genomics. doi: 10.1016/j.jgg.2023.09.002
Citation: Yaling Wang, Xueying Huang, Guoying Sun, Jingwen Chen, Bangguo Wu, Jiahui Luo, Shuyan Tang, Peng Dai, Feng Zhang, Jinsong Li, Lingbo Wang. Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice[J]. Journal of Genetics and Genomics. doi: 10.1016/j.jgg.2023.09.002
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Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice

doi: 10.1016/j.jgg.2023.09.002

  • a State Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Metabolic Remodeling and Health, Institute of Metabolism and Integrative Biology, Fudan University, Shanghai 200438, China;

  • b Institute of Reproduction and Development, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China;

  • c Shanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China;

  • d State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China

基金项目: 

This work was supported by the National Key Research and Development Program of China (2021YFC2701400) and in part by the National Natural Science Foundation of China (32000393).

详细信息
    通讯作者:

    Lingbo Wang,E-mail address:wanglingbo@fudan.edu.cn

Coiled-coil domain-containing 38 is required for acrosome biogenesis and fibrous sheath assembly in mice

  • a State Key Laboratory of Genetic Engineering, Shanghai Key Laboratory of Metabolic Remodeling and Health, Institute of Metabolism and Integrative Biology, Fudan University, Shanghai 200438, China;

  • b Institute of Reproduction and Development, Obstetrics and Gynecology Hospital, Fudan University, Shanghai 200011, China;

  • c Shanghai Key Laboratory of Maternal and Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 200092, China;

  • d State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China

Funds: 

This work was supported by the National Key Research and Development Program of China (2021YFC2701400) and in part by the National Natural Science Foundation of China (32000393).

    摘要
  • 摘要: During spermiogenesis, haploid spermatids undergo dramatic morphological changes to form slender sperm flagella and cap-like acrosomes, which are required for successful fertilization. Severe deformities in flagella cause a male infertility syndrome, multiple morphological abnormalities of the flagella (MMAF), while acrosomal hypoplasia in some cases leads to sub-optimal embryonic developmental potential. However, evidence regarding the occurrence of acrosomal hypoplasia in MMAF is limited. Here, we report the generation of base-edited mice knocked out for coiled-coil domain-containing 38 (Ccdc38) via inducing a nonsense mutation and find that the males are infertile. The Ccdc38-KO sperm display acrosomal hypoplasia and typical MMAF phenotypes. We find that the acrosomal membrane is loosely anchored to the nucleus and fibrous sheaths are disorganized in Ccdc38-KO sperm. Further analyses reveal that Ccdc38 knockout causes a decreased level of TEKT3, a protein associated with acrosome biogenesis, in testes and an aberrant distribution of TEKT3 on sperm. We finally show that intracytoplasmic sperm injection overcomes Ccdc38-related infertility. Our study thus reveals a previously unknown role for CCDC38 in acrosome biogenesis and provides additional evidence for the occurrence of acrosomal hypoplasia in MMAF.
    Abstract: During spermiogenesis, haploid spermatids undergo dramatic morphological changes to form slender sperm flagella and cap-like acrosomes, which are required for successful fertilization. Severe deformities in flagella cause a male infertility syndrome, multiple morphological abnormalities of the flagella (MMAF), while acrosomal hypoplasia in some cases leads to sub-optimal embryonic developmental potential. However, evidence regarding the occurrence of acrosomal hypoplasia in MMAF is limited. Here, we report the generation of base-edited mice knocked out for coiled-coil domain-containing 38 (Ccdc38) via inducing a nonsense mutation and find that the males are infertile. The Ccdc38-KO sperm display acrosomal hypoplasia and typical MMAF phenotypes. We find that the acrosomal membrane is loosely anchored to the nucleus and fibrous sheaths are disorganized in Ccdc38-KO sperm. Further analyses reveal that Ccdc38 knockout causes a decreased level of TEKT3, a protein associated with acrosome biogenesis, in testes and an aberrant distribution of TEKT3 on sperm. We finally show that intracytoplasmic sperm injection overcomes Ccdc38-related infertility. Our study thus reveals a previously unknown role for CCDC38 in acrosome biogenesis and provides additional evidence for the occurrence of acrosomal hypoplasia in MMAF.
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  • 收稿日期:  2023-05-23
  • 修回日期:  2023-09-06
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