Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content

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Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content

Jing Zhu, Qing Chen, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Lu Lei, Caihong Liu, Yue Li, Rui Tang, Jie Tang, Ziyi Zhang, Shijing Peng, Mi Zhang, Zhongxu Chen, Li Kong, Mei Deng, Qiang Xu, Yazhou Zhang, Qiantao Jiang, Jirui Wang, Guoyue Chen, Yunfeng Jiang, Yuming Wei, Youliang Zheng, Pengfei Qi

文章导航 > Journal of Genetics and Genomics > 2025 > 优先出版

Jing Zhu, Qing Chen, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Lu Lei, Caihong Liu, Yue Li, Rui Tang, Jie Tang, Ziyi Zhang, Shijing Peng, Mi Zhang, Zhongxu Chen, Li Kong, Mei Deng, Qiang Xu, Yazhou Zhang, Qiantao Jiang, Jirui Wang, Guoyue Chen, Yunfeng Jiang, Yuming Wei, Youliang Zheng, Pengfei Qi. Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content[J]. 遗传学报. doi: 10.1016/j.jgg.2025.02.011

引用本文:

Jing Zhu, Qing Chen, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Lu Lei, Caihong Liu, Yue Li, Rui Tang, Jie Tang, Ziyi Zhang, Shijing Peng, Mi Zhang, Zhongxu Chen, Li Kong, Mei Deng, Qiang Xu, Yazhou Zhang, Qiantao Jiang, Jirui Wang, Guoyue Chen, Yunfeng Jiang, Yuming Wei, Youliang Zheng, Pengfei Qi. Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content[J]. 遗传学报. doi: 10.1016/j.jgg.2025.02.011

Jing Zhu, Qing Chen, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Lu Lei, Caihong Liu, Yue Li, Rui Tang, Jie Tang, Ziyi Zhang, Shijing Peng, Mi Zhang, Zhongxu Chen, Li Kong, Mei Deng, Qiang Xu, Yazhou Zhang, Qiantao Jiang, Jirui Wang, Guoyue Chen, Yunfeng Jiang, Yuming Wei, Youliang Zheng, Pengfei Qi. Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content[J]. Journal of Genetics and Genomics. doi: 10.1016/j.jgg.2025.02.011

Citation:

Jing Zhu, Qing Chen, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Lu Lei, Caihong Liu, Yue Li, Rui Tang, Jie Tang, Ziyi Zhang, Shijing Peng, Mi Zhang, Zhongxu Chen, Li Kong, Mei Deng, Qiang Xu, Yazhou Zhang, Qiantao Jiang, Jirui Wang, Guoyue Chen, Yunfeng Jiang, Yuming Wei, Youliang Zheng, Pengfei Qi. Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content[J]. Journal of Genetics and Genomics. doi: 10.1016/j.jgg.2025.02.011

Jing Zhu, Qing Chen, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Lu Lei, Caihong Liu, Yue Li, Rui Tang, Jie Tang, Ziyi Zhang, Shijing Peng, Mi Zhang, Zhongxu Chen, Li Kong, Mei Deng, Qiang Xu, Yazhou Zhang, Qiantao Jiang, Jirui Wang, Guoyue Chen, Yunfeng Jiang, Yuming Wei, Youliang Zheng, Pengfei Qi. Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content[J]. 遗传学报. doi: 10.1016/j.jgg.2025.02.011

引用本文:

Jing Zhu, Qing Chen, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Lu Lei, Caihong Liu, Yue Li, Rui Tang, Jie Tang, Ziyi Zhang, Shijing Peng, Mi Zhang, Zhongxu Chen, Li Kong, Mei Deng, Qiang Xu, Yazhou Zhang, Qiantao Jiang, Jirui Wang, Guoyue Chen, Yunfeng Jiang, Yuming Wei, Youliang Zheng, Pengfei Qi. Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content[J]. 遗传学报. doi: 10.1016/j.jgg.2025.02.011

Jing Zhu, Qing Chen, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Lu Lei, Caihong Liu, Yue Li, Rui Tang, Jie Tang, Ziyi Zhang, Shijing Peng, Mi Zhang, Zhongxu Chen, Li Kong, Mei Deng, Qiang Xu, Yazhou Zhang, Qiantao Jiang, Jirui Wang, Guoyue Chen, Yunfeng Jiang, Yuming Wei, Youliang Zheng, Pengfei Qi. Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content[J]. Journal of Genetics and Genomics. doi: 10.1016/j.jgg.2025.02.011

Citation:

Jing Zhu, Qing Chen, Zhenru Guo, Yan Wang, Qingcheng Li, Yang Li, Lu Lei, Caihong Liu, Yue Li, Rui Tang, Jie Tang, Ziyi Zhang, Shijing Peng, Mi Zhang, Zhongxu Chen, Li Kong, Mei Deng, Qiang Xu, Yazhou Zhang, Qiantao Jiang, Jirui Wang, Guoyue Chen, Yunfeng Jiang, Yuming Wei, Youliang Zheng, Pengfei Qi. Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content[J]. Journal of Genetics and Genomics. doi: 10.1016/j.jgg.2025.02.011

Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content

doi: 10.1016/j.jgg.2025.02.011

a. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, Sichuan 611130, China;
b. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China

基金项目:

This study was supported by the National Key Research and Development Program of China (2023YFD1200404), the Science and Technology Department of Sichuan Province (2024ZYD0160), and the National Natural Science Foundation of China (32072054). We thank Liwen Bianji (Edanz) (www.liwenbianji.cn) for editing the English text of a draft of this manuscript. Bioinformatics analysis was supported by the high-performance computing platform of Sichuan Agricultural University.

通讯作者:
Yuming Wei,E-mail:ymwei@sicau.edu.cn

Pengfei Qi,E-mail:pengfeiqi@hotmail.com

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出版历程
- 收稿日期: 2024-11-05
- 录用日期: 2025-02-20
- 修回日期: 2025-02-19
- 网络出版日期: 2025-07-11

Genome-wide analysis of Q binding reveals a regulatory network that coordinates wheat grain yield and grain protein content

a. State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Chengdu, Sichuan 611130, China;
b. Triticeae Research Institute, Sichuan Agricultural University, Chengdu, Sichuan 611130, China

Funds:

This study was supported by the National Key Research and Development Program of China (2023YFD1200404), the Science and Technology Department of Sichuan Province (2024ZYD0160), and the National Natural Science Foundation of China (32072054). We thank Liwen Bianji (Edanz) (www.liwenbianji.cn) for editing the English text of a draft of this manuscript. Bioinformatics analysis was supported by the high-performance computing platform of Sichuan Agricultural University.

摘要:
Wheat is an important cereal crop used to produce diverse and popular food worldwide because of its high grain yield (GY) and grain protein content (GPC). However, GY and GPC are usually negatively correlated. We previously reported that favorable alleles of the wheat domestication gene Q can synchronously increase GY and GPC, but the underlying mechanisms remain largely unknown. In this study, we investigated the regulatory network involving Q associated with GY and GPC in young grains through DNA affinity purification sequencing and transcriptome sequencing analyses, electrophoretic mobility shift and dual-luciferase assays, and transgenic approaches. Three Q-binding motifs, namely TTAAGG, AAACA[A/T]A, and GTAC[T/G]A, were identified. Notably, genes related to photosynthesis or carbon and nitrogen metabolism were enriched and regulated by Q. Moreover, Q was revealed to bind directly to its own gene and the glutamine synthetase gene GSr-4D to increase expression, thereby influencing nitrogen assimilation during the grain filling stage and increasing GPC. Considered together, our study findings provide molecular evidence of the positive regulatory effects of Q on wheat GY and GPC.
- Grain yield /
- Grain protein content /
- Q gene /
- Glutamine synthetase /
- Mechanism /
- Wheat
Abstract:
Wheat is an important cereal crop used to produce diverse and popular food worldwide because of its high grain yield (GY) and grain protein content (GPC). However, GY and GPC are usually negatively correlated. We previously reported that favorable alleles of the wheat domestication gene Q can synchronously increase GY and GPC, but the underlying mechanisms remain largely unknown. In this study, we investigated the regulatory network involving Q associated with GY and GPC in young grains through DNA affinity purification sequencing and transcriptome sequencing analyses, electrophoretic mobility shift and dual-luciferase assays, and transgenic approaches. Three Q-binding motifs, namely TTAAGG, AAACA[A/T]A, and GTAC[T/G]A, were identified. Notably, genes related to photosynthesis or carbon and nitrogen metabolism were enriched and regulated by Q. Moreover, Q was revealed to bind directly to its own gene and the glutamine synthetase gene GSr-4D to increase expression, thereby influencing nitrogen assimilation during the grain filling stage and increasing GPC. Considered together, our study findings provide molecular evidence of the positive regulatory effects of Q on wheat GY and GPC.
- Grain yield /
- Grain protein content /
- Q gene /
- Glutamine synthetase /
- Mechanism /
- Wheat

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