DNA methylation landscapes of in vitro matured oocytes retrieved during endoscopic gynaecological procedures

Cui-Ling Lu; Xue-Ling Song; Xiao-Ying Zheng; Tian-Shu Song; Xiao-Na Wang; Jie Yan; Rui Yang; Rong Li; Jie Qiao

doi:10.1016/j.jgg.2025.05.002

DNA methylation landscapes of in vitro matured oocytes retrieved during endoscopic gynaecological procedures

doi: 10.1016/j.jgg.2025.05.002

a. Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China;
b. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;
c. Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

基金项目:

This work was supported by funding of National Natural Science Foundation of China (81971349 and 81300456). We are deeply grateful to Dr. Zhu-Qiang Zhang (Institute of Biophysics, Chinese Academy of Sciences) for his assistance with rewriting this paper and data analysis, thank Dr. Hong-Shan Guo (Zhejiang University School of Medicine) for his methylation-seq data of human oocyte maturation matured in vivo and technical guidance, and thank Dr. Wei Chen (Peking University Third Hospital) for his assistance with data analysis. Additionally, we thank all the doctors and nurses of the Reproductive Medical Centre of Peking University Third Hospital for their excellent assistance.

详细信息

通讯作者:
Cui-Ling Lu,E-mail:luclbj@ibp.ac.cn

Rui Yang,E-mail:yrjeff@126.com

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出版历程
- 收稿日期: 2025-03-04
- 录用日期: 2025-05-06
- 修回日期: 2025-04-30
- 网络出版日期: 2025-07-11

DNA methylation landscapes of in vitro matured oocytes retrieved during endoscopic gynaecological procedures

a. Center for Reproductive Medicine, Peking University Third Hospital, Beijing 100191, China;
b. National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China;
c. Key Laboratory of Epigenetic Regulation and Intervention, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China

Funds:

摘要

摘要:
In vitro maturation (IVM) of human oocytes offers cost efficiency and minimal invasiveness, serving as a valuable supplementary tool in assisted reproduction for fertility preservation, ovarian hyperstimulation syndrome prevention, and other reproductive strategies. Despite its availability for three decades, the clinical use of IVM remains limited due to efficacy and safety concerns. This study examines the DNA methylation profile of IVM oocytes collected during laparoscopic/hysteroscopic surgeries compared to in vivo matured oocytes via reduced representation bisulfite sequencing. Results indicate IVM oocytes exhibit a higher global methylation level. Differentially methylated regions (DMR) analysis reveals that the in vitro group displays more hypermethylated and fewer hypomethylated DMRs compared to the in vivo group. Additionally, the in vitro group exhibits a higher level of non-CpG methylation than the in vivo group. However, no significant correlation between methylation levels and transcriptional activity in these oocytes is found, especially for those specific imprinted genes or genes related to embryonic development. These findings shed light on the epigenetic landscape of IVM oocytes, contributing to the ongoing assessment of their clinical feasibility and safety in assisted reproduction.
- In vitro maturation (IVM) /
- DNA methylation /
- Reduced representation bisulfite sequencing (RRBS) /
- Differentially methylated regions (DMR) /
- Oocyte
Abstract:
In vitro maturation (IVM) of human oocytes offers cost efficiency and minimal invasiveness, serving as a valuable supplementary tool in assisted reproduction for fertility preservation, ovarian hyperstimulation syndrome prevention, and other reproductive strategies. Despite its availability for three decades, the clinical use of IVM remains limited due to efficacy and safety concerns. This study examines the DNA methylation profile of IVM oocytes collected during laparoscopic/hysteroscopic surgeries compared to in vivo matured oocytes via reduced representation bisulfite sequencing. Results indicate IVM oocytes exhibit a higher global methylation level. Differentially methylated regions (DMR) analysis reveals that the in vitro group displays more hypermethylated and fewer hypomethylated DMRs compared to the in vivo group. Additionally, the in vitro group exhibits a higher level of non-CpG methylation than the in vivo group. However, no significant correlation between methylation levels and transcriptional activity in these oocytes is found, especially for those specific imprinted genes or genes related to embryonic development. These findings shed light on the epigenetic landscape of IVM oocytes, contributing to the ongoing assessment of their clinical feasibility and safety in assisted reproduction.
- In vitro maturation (IVM) /
- DNA methylation /
- Reduced representation bisulfite sequencing (RRBS) /
- Differentially methylated regions (DMR) /
- Oocyte

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DNA methylation landscapes of in vitro matured oocytes retrieved during endoscopic gynaecological procedures

doi: 10.1016/j.jgg.2025.05.002

通讯作者:
Cui-Ling Lu,E-mail:luclbj@ibp.ac.cn

Rui Yang,E-mail:yrjeff@126.com

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DNA methylation landscapes of in vitro matured oocytes retrieved during endoscopic gynaecological procedures

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DNA methylation landscapes of in vitro matured oocytes retrieved during endoscopic gynaecological procedures

doi: 10.1016/j.jgg.2025.05.002

通讯作者: Cui-Ling Lu,E-mail:luclbj@ibp.ac.cn Rui Yang,E-mail:yrjeff@126.com

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出版历程

DNA methylation landscapes of in vitro matured oocytes retrieved during endoscopic gynaecological procedures

计量

出版历程

目录

通讯作者:
Cui-Ling Lu,E-mail:luclbj@ibp.ac.cn

Rui Yang,E-mail:yrjeff@126.com