<i>Xgr</i> is involved in body size control in <i>Drosophila</i> through promoting glucose uptake in the Malpighian tubules

Zhiwei Lin; Zihao He; Jianfeng Guo; Xiaofang Ji; Ze Hu; Yingsen Tang; Chuanxian Wei; Jiyong Liu; Wenqi Wu; Jun Ma; Renjie Jiao

doi:10.1016/j.jgg.2025.05.007

Xgr is involved in body size control in Drosophila through promoting glucose uptake in the Malpighian tubules

doi: 10.1016/j.jgg.2025.05.007

a. Sino-French Hoffmann Institute, Guangdong Provincial Key Laboratory of Protein Modification and Degradation, School of Basic Medical Sciences, Second Affiliated Hospital, Guangzhou Medical University, 1 Xinzao Road, Guangzhou 511436, China;
b. Center for Genetic Medicine, the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China;
c. Institute of Genetics, International School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China

基金项目:

to Dr. Suning Liu for AMPK-T184D flies. This study was funded by the National Key R&D Program of China (2021YFA0805800, 2023YFE0107700, and 2020YFA0803202 to R.J.), the 111 Project (D18010 to R.J.), the Guangzhou Medical University Discipline Construction Funds (Basic Medicine) (JCXKJS2022A02 to R.J.), Guangdong Basic and Applied Basic Research Foundation (2024A1515010752 to C.W.), and the Special Innovation Projects of Universities in Guangdong Province (2022KTSCX096 to C.W.). Thanks to the Jiao lab members for stimulating discussions.

We thank the BDSC, VDRC, THFC, and GDRC for providing fly stocks. We are grateful to Dr. Hai Huang for ATP-SPARK and AMPK-SPARK flies

详细信息

通讯作者:
Renjie Jiao,E-mail:rjiao@gzhmu.edu.cn

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出版历程
- 收稿日期: 2025-03-01
- 录用日期: 2025-05-15
- 修回日期: 2025-05-15
- 网络出版日期: 2025-07-11

Xgr is involved in body size control in Drosophila through promoting glucose uptake in the Malpighian tubules

a. Sino-French Hoffmann Institute, Guangdong Provincial Key Laboratory of Protein Modification and Degradation, School of Basic Medical Sciences, Second Affiliated Hospital, Guangzhou Medical University, 1 Xinzao Road, Guangzhou 511436, China;
b. Center for Genetic Medicine, the Fourth Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310058, China;
c. Institute of Genetics, International School of Medicine, Zhejiang University, Hangzhou, Zhejiang 310058, China

Funds:

We thank the BDSC, VDRC, THFC, and GDRC for providing fly stocks. We are grateful to Dr. Hai Huang for ATP-SPARK and AMPK-SPARK flies

摘要

摘要:
Body size control is fundamental to development and requires proper energy engagement. One of the key energy sensing factors is AMP-activated protein kinase (AMPK), which regulates glucose uptake to ensure ATP production and nutrition supply during development. Here, we identify that the mutation of xgr, a gene encoding an ATPase, results in a reduced body size in Drosophila. Xgr is primarily expressed in the epithelial cells of the Malpighian tubules and the midguts. Loss of xgr leads to the inactivation of the AMPK signaling due to an increased ATP level. Glucose reabsorption in the Malpighian tubules is significantly reduced, as the Glut1 translocation to the plasma membrane is significantly disrupted in the absence of Xgr function. Our results suggest that Xgr function in the Malpighian tubules is essential to systemic glucose supply and energy homeostasis at the organismal level, thereby impacting body size. Our findings provide a mechanistic connection between energy homeostasis and animal size control during development.
- Drosophila /
- ATPase /
- Glut1 /
- AMPK /
- Development /
- Glucose uptake
Abstract:
Body size control is fundamental to development and requires proper energy engagement. One of the key energy sensing factors is AMP-activated protein kinase (AMPK), which regulates glucose uptake to ensure ATP production and nutrition supply during development. Here, we identify that the mutation of xgr, a gene encoding an ATPase, results in a reduced body size in Drosophila. Xgr is primarily expressed in the epithelial cells of the Malpighian tubules and the midguts. Loss of xgr leads to the inactivation of the AMPK signaling due to an increased ATP level. Glucose reabsorption in the Malpighian tubules is significantly reduced, as the Glut1 translocation to the plasma membrane is significantly disrupted in the absence of Xgr function. Our results suggest that Xgr function in the Malpighian tubules is essential to systemic glucose supply and energy homeostasis at the organismal level, thereby impacting body size. Our findings provide a mechanistic connection between energy homeostasis and animal size control during development.
- Drosophila /
- ATPase /
- Glut1 /
- AMPK /
- Development /
- Glucose uptake

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Xgr is involved in body size control in Drosophila through promoting glucose uptake in the Malpighian tubules

doi: 10.1016/j.jgg.2025.05.007

通讯作者:
Renjie Jiao,E-mail:rjiao@gzhmu.edu.cn

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Xgr is involved in body size control in Drosophila through promoting glucose uptake in the Malpighian tubules

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Xgr is involved in body size control in Drosophila through promoting glucose uptake in the Malpighian tubules

doi: 10.1016/j.jgg.2025.05.007

通讯作者: Renjie Jiao,E-mail:rjiao@gzhmu.edu.cn

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出版历程

Xgr is involved in body size control in Drosophila through promoting glucose uptake in the Malpighian tubules

计量

出版历程

目录

通讯作者:
Renjie Jiao,E-mail:rjiao@gzhmu.edu.cn