Proximity labeling proteomics with cilia-TurboID transgenic mice identified regulators of motile cilia function

Pan Wang; Xiangnan Wu; Liqing Xiao; Qingru Yu; Yan Peng; Mengting Yan; Jun Tang; Mingqiang Hu; Hongtao Li; Li Li; Lingfei Luo; Ming Ma

doi:10.1016/j.jgg.2025.07.013

Proximity labeling proteomics with cilia-TurboID transgenic mice identified regulators of motile cilia function

doi: 10.1016/j.jgg.2025.07.013

1. Institute of Developmental Biology and Regenerative Medicine, Southwest University, Chongqing 400715, China;
2. Henan Provincial Reproductive Obstetrics and Gynecology Hospital, Henan Provincial People's Hospital, Institute of Reproductive Health, Henan Academy of Innovations in Medical Science, Henan 450003, China;
3. School of Life Sciences, Southwest University, Chongqing 400715, China;
4. Research center of Stem cells and Ageing, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China;
5. School of Life Sciences, Fudan University, Shanghai 200438, China

基金项目:

Henan Key Laboratory of Population Defects Prevention (ZD202302).

This work was supported by grants from the National Key Research and Development Program of China (2019YFA0802704), the National Natural Science Foundation of China (31771620), the Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-MSX1424), Research Startup Fund of Southwest University (SWU117064), and Open Research Fund of National Health Commission Key Laboratory of Birth Defects Prevention &

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通讯作者:
Ming Ma,E-mail:skymaming@swu.edu.cn

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- 收稿日期: 2025-01-22
- 录用日期: 2025-07-28
- 修回日期: 2025-07-28
- 网络出版日期: 2025-08-06

Proximity labeling proteomics with cilia-TurboID transgenic mice identified regulators of motile cilia function

1. Institute of Developmental Biology and Regenerative Medicine, Southwest University, Chongqing 400715, China;
2. Henan Provincial Reproductive Obstetrics and Gynecology Hospital, Henan Provincial People's Hospital, Institute of Reproductive Health, Henan Academy of Innovations in Medical Science, Henan 450003, China;
3. School of Life Sciences, Southwest University, Chongqing 400715, China;
4. Research center of Stem cells and Ageing, Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Sciences, Chongqing 400714, China;
5. School of Life Sciences, Fudan University, Shanghai 200438, China

Funds:

Henan Key Laboratory of Population Defects Prevention (ZD202302).

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参考文献(13)

Cho, K.F., Branon, T.C., Udeshi, N.D., Myers, S.A., Carr, S.A., Ting, A.Y., 2020. Proximity labeling in mammalian cells with TurboID and split-TurboID. Nat. Protoc. 15, 3971-3999.

Fernandez-Gonzalez, A., Kourembanas, S., Wyatt, T.A., Mitsialis, S.A., 2009. Mutation of murine adenylate kinase 7 underlies a primary ciliary dyskinesia phenotype. Am. J. Respir. Cell Mol. Biol. 40, 305-313.

Ishikawa, H., Thompson, J., Yates, J.R., 3rd, Marshall, W.F., 2012. Proteomic analysis of mammalian primary cilia. Curr. Biol. 22, 414-419.

Liu, X., Yam, P.T., Schlienger, S., Cai, E., Zhang, J., Chen, W.J., Torres Gutierrez, O., Jimenez Amilburu, V., Ramamurthy, V., Ting, A.Y., et al., 2024. Numb positively regulates Hedgehog signaling at the ciliary pocket. Nat. Commun. 15, 3365.

Loukil, A., Ebright, E., Hamdi, K., Menzel, E., Uezu, A., Gao, Y., Soderling, S.H., Goetz, S.C., 2025. Identification of new ciliary signaling pathways in the brain and insights into neurological disorders. J. Neurosci. https://doi.org/10.1523/JNEUROSCI.0800-24.2025.

Martino, F., Pal, M., Munoz-Hernandez, H., Rodriguez, C.F., Nunez-Ramirez, R., Gil-Carton, D., Degliesposti, G., Skehel, J.M., Roe, S.M., Prodromou, C., et al., 2018. RPAP3 provides a flexible scaffold for coupling HSP90 to the human R2TP co-chaperone complex. Nat. Commun. 9, 1501.

Mick, D.U., Rodrigues, R.B., Leib, R.D., Adams, C.M., Chien, A.S., Gygi, S.P., Nachury, M.V., 2015. Proteomics of primary cilia by proximity labeling. Dev. Cell 35, 497-512.

Murata, K., Mimura, A., Suzuki, H., Mikami, N., Hamada, Y., Kato, K., Iki, N., Ishida, M., Daitoku, Y., Tanimoto, Y., et al., 2021. Efficient induction of proximity-dependent labelling by biotin feeding in BMAL1-BioID knock-in mice. J. Biochem. 170, 453-461.

Reiter, J.F., Leroux, M.R., 2017. Genes and molecular pathways underpinning ciliopathies. Nat. Rev. Mol. Cell Biol. 18, 533-547.

Sheridan, J., Grata, A., Dorr, J., Suva, E.E., Bresteau, E., Mitchell, L.R., Hassan, O., Mitchell, B., 2025. Centriolar defects underlie a primary ciliary dyskinesia phenotype in an adenylate kinase 7 deficient ciliated epithelium. Dev. Biol. 524, 152-161.

Sigg, M.A., Menchen, T., Lee, C., Johnson, J., Jungnickel, M.K., Choksi, S.P., Garcia, G., Busengdal, H., Dougherty, G.W., Pennekamp, P., 2017. Evolutionary proteomics uncovers ancient associations of cilia with signaling pathways. Dev. Cell 43, 744-762.

Wallmeier, J., Nielsen, K.G., Kuehni, C.E., Lucas, J.S., Leigh, M.W., Zariwala, M.A., Omran, H., 2020. Motile ciliopathies. Nat. Rev. Dis. Primers 6, 77.

Wu, H., Zhang, Y., Li, Y., Sun, S., Zhang, J., Xie, Q., Dong, Y., Zhou, S., Sha, X., Li, K., et al., 2024. Adenylate kinase phosphate energy shuttle underlies energetic communication in flagellar axonemes. Sci. China Life Sci. 67, 1697-1714.

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Proximity labeling proteomics with cilia-TurboID transgenic mice identified regulators of motile cilia function

doi: 10.1016/j.jgg.2025.07.013

通讯作者:
Ming Ma,E-mail:skymaming@swu.edu.cn

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Proximity labeling proteomics with cilia-TurboID transgenic mice identified regulators of motile cilia function

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Proximity labeling proteomics with cilia-TurboID transgenic mice identified regulators of motile cilia function

doi: 10.1016/j.jgg.2025.07.013

通讯作者: Ming Ma,E-mail:skymaming@swu.edu.cn

计量

出版历程

Proximity labeling proteomics with cilia-TurboID transgenic mice identified regulators of motile cilia function

计量

出版历程

目录

通讯作者:
Ming Ma,E-mail:skymaming@swu.edu.cn