Retarded Embryo Development 1 (RED1) regulates embryo development, seed maturation and plant growth in Arabidopsis

Qian Du; Huanzhong Wang

doi:10.1016/j.jgg.2016.04.009

Volume 43 Issue 7

Jul. 2016

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Retarded Embryo Development 1 (RED1) regulates embryo development, seed maturation and plant growth in Arabidopsis

doi: 10.1016/j.jgg.2016.04.009

Qian Du^a,
Huanzhong Wang^{a, b}

a
Department of Plant Science and Landscape Architecture, University of Connecticut, Storrs, CT 06269, USA
b
Institute for Systems Genomics, University of Connecticut, Storrs, CT 06269, USA

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Corresponding author: E-mail address: huanzhong.wang@uconn.edu (Huanzhong Wang)
Received Date: 2015-09-03
Accepted Date: 2016-04-23
Rev Recd Date: 2016-04-14

Available Online: 2016-04-24

Publish Date: 2016-07-20

Abstract

Abstract

Plant seeds accumulate large amounts of protein and carbohydrate as storage reserves during maturation. Thus, understanding the genetic control of embryo and seed development may provide bioengineering tools for yield improvement. In this study, we report the identification of Retarded Embryo Development 1 (RED1) gene in Arabidopsis, whose two independent T-DNA insertion mutant lines, SALK_085642 (red1-1) and SALK_022583 (red1-2), show a retarded embryo development phenotype. The embryogenesis process ceases at the late heart stage in red1-1 and at the bent-cotyledon stage in red1-2, respectively, resulting in seed abortion in both lines. The retarded embryo development and seed abortion phenotypes reverted to normal when RED1 complementation constructs were introduced into mutant plants. Small red1-2 homozygous plants can be successfully rescued by culturing immature seeds, indicating that seed abortion likely results from compromised tolerance to the desiccation process associated with seed maturation. Consistent with this observation, red1-2 seeds accumulate less protein, and the expression of two late embryo development reporter transgenes, LEA::GUS and β-conglycinin::GUS, was significantly weak and started relatively late in the red1-2 mutant lines compared to the wild type. The RED1 gene encodes a plant specific novel protein that is localized in the nucleus. These results indicate that RED1 plays important roles in embryo development, seed maturation and plant growth.
- Embryo,
- Development,
- Seed maturation,
- Growth

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References(56)

References

[1]	Alandete-Saez, M., Ron, M., McCormick, S. Mol. Plant, 1 (2008),pp. 586-598
[2]	Alonso, J.M., Stepanova, A.N., Leisse, T.J. et al. Science, 301 (2003),pp. 653-657
[3]	Baud, S., Boutin, J.P., Miquel, M. et al. Plant Physiol. Bioch., 40 (2002),pp. 151-160
[4]	Baud, S., Mendoza, M.S., To, A. et al. Plant J., 50 (2007),pp. 825-838
[5]	Baumlein, H., Misera, S., Luerssen, H. et al. Plant J., 6 (1994),pp. 379-387
[6]	Berleth, T., Chatfield, S.
[7]	Braybrook, S.A., Harada, J.J. LECs go crazy in embryo development Trends Plant Sci., 13 (2008),pp. 624-630
[8]	Braybrook, S.A., Stone, S.L., Park, S. et al. Genes directly regulated by LEAFY COTYLEDON2 provide insight into the control of embryo maturation and somatic embryogenesis Proc. Natl. Acad. Sci. U. S. A., 103 (2006),pp. 3468-3473
[9]	Cernac, A., Benning, C. Plant J., 40 (2004),pp. 575-585
[10]	Clough, S.J., Bent, A.F. Plant J., 16 (1998),pp. 735-743
[11]	De Smet, I., Lau, S., Mayer, U. et al. Embryogenesis - the humble beginnings of plant life Plant J., 61 (2010),pp. 959-970
[12]	Engel, M.L., Chaboud, A., Dumas, C. et al. Plant J., 34 (2003),pp. 697-707
[13]	Gazzarrini, S., Tsuchiya, Y., Lumba, S. et al. Dev. Cell, 7 (2004),pp. 373-385
[14]	Giraudat, J., Hauge, B.M., Valon, C. et al. Plant Cell, 4 (1992),pp. 1251-1261
[15]	Goldberg, R.B., de Paiva, G., Yadegari, R. Plant embryogenesis: zygote to seed Science, 266 (1994),pp. 605-614
[16]	Gomez, L.D., Baud, S., Gilday, A. et al. Plant J., 46 (2006),pp. 69-84
[17]	Goupil, P., Hatzopoulos, P., Franz, G. et al. Plant Mol. Biol., 18 (1992),pp. 1049-1063
[18]	Gutierrez, L., Van Wuytswinkel, O., Castelain, M. et al. Combined networks regulating seed maturation Trends Plant Sci., 12 (2007),pp. 294-300
[19]	Holdsworth, M., Kurup, S., McKibbin, R. Molecular and genetic mechanisms regulating the transition from embryo development to germination Trends Plant Sci., 4 (1999),pp. 275-280
[20]	Johnson, M.A., Preuss, D. Plotting a course: multiple signals guide pollen tubes to their targets Dev. Cell, 2 (2002),pp. 273-281
[21]	Karimi, M., Inze, D., Depicker, A. Trends Plant Sci., 7 (2002),pp. 193-195
[22]	Keith, K., Kraml, M., Dengler, N.G. et al. Plant Cell, 6 (1994),pp. 589-600
[23]	Kondou, Y., Nakazawa, M., Kawashima, M. et al. RETARDED GROWTH OF EMBRYO1, a new basic helix-loop-helix protein, expresses in endosperm to control embryo growth Plant Physiol., 147 (2008),pp. 1924-1935
[24]	Koornneef, M., Reuling, G., Karssen, C.M. Physiol. Plant., 61 (1984),pp. 377-383
[25]	Kroj, T., Savino, G., Valon, C. et al. Development, 130 (2003),pp. 6065-6073
[26]	Larkin, M.A., Blackshields, G., Brown, N.P. et al. Clustal W and clustal X version 2.0 Bioinformatics, 23 (2007),pp. 2947-2948
[27]	Laux, T., Jurgens, G. Embryogenesis: a new start in life Plant Cell, 9 (1997),pp. 989-1000
[28]	Laux, T., Wurschum, T., Breuninger, H. Genetic regulation of embryonic pattern formation Plant Cell, 16 (2004),pp. S190-S202
[29]	Lee, H., Fischer, R.L., Goldberg, R.B. et al. Proc. Natl. Acad. Sci. U. S. A., 100 (2003),pp. 2152-2156
[30]	Lotan, T., Ohto, M., Yee, K.M. et al. Cell, 93 (1998),pp. 1195-1205
[31]	Luerssen, H., Kirik, V., Herrmann, P. et al. Plant J., 15 (1998),pp. 755-764
[32]	Manfre, A.J., LaHatte, G.A., Climer, C.R. et al. Plant Cell Physiol., 50 (2009),pp. 243-253
[33]	Masaki, T., Mitsui, N., Tsukagoshi, H. et al. Plant Cell Physiol., 46 (2005),pp. 547-556
[34]	Mayer, U., Ruiz, R.A.T., Berleth, T. et al. Nature, 353 (1991),pp. 402-407
[35]	Meinke, D.W. Science, 258 (1992),pp. 1647-1650
[36]	Meinke, D.W., Franzmann, L.H., Nickle, T.C. et al. Plant Cell, 6 (1994),pp. 1049-1064
[37]	Mietkiewska, E., Giblin, E.M., Wang, S. et al. Plant Physiol., 136 (2004),pp. 2665-2675
[38]	Monke, G., Altschmied, L., Tewes, A. et al. Seed-specific transcription factors ABI3 and FUS3: molecular interaction with DNA Planta, 219 (2004),pp. 158-166
[39]	Naito, S., Hirai, M.Y., Chino, M. et al. Plant Physiol., 104 (1994),pp. 497-503
[40]	North, H., Baud, S., Debeaujon, I. et al. Plant J., 61 (2010),pp. 971-981
[41]	Rawsthorne, S. Carbon flux and fatty acid synthesis in plants Prog. Lipid Res., 41 (2002),pp. 182-196
[42]	Reidt, W., Wohlfarth, T., Ellerstrom, M. et al. Plant J., 21 (2000),pp. 401-408
[43]	Rohde, A., De Rycke, R., Beeckman, T. et al. Plant Cell, 12 (2000),pp. 35-52
[44]	Santos-Mendoza, M., Dubreucq, B., Baud, S. et al. Plant J., 54 (2008),pp. 608-620
[45]	Savage, L.J., Imre, K.M., Hall, D.A. et al. PLoS One, 8 (2013),p. e73291
[46]	Stone, S.L., Kwong, L.W., Yee, K.M. et al. Proc. Natl. Acad. Sci. U. S. A., 98 (2001),pp. 11806-11811
[47]	Sunilkumar, G., Mohr, L., Lopata-Finch, E. et al. Developmental and tissue-specific expression of CaMV 35S promoter in cotton as revealed by GFP Plant Mol. Biol., 50 (2002),pp. 463-474
[48]	To, A., Valon, C., Savino, G. et al. Plant Cell, 18 (2006),pp. 1642-1651
[49]	Tsuchiya, Y., Nambara, E., Naito, S. et al. Plant J., 37 (2004),pp. 73-81
[50]	Tsukagoshi, H., Morikami, A., Nakamura, K. Proc. Natl. Acad. Sci. U. S. A., 104 (2007),pp. 2543-2547
[51]	Tzafrir, I., Pena-Muralla, R., Dickerman, A. et al. Plant Physiol., 135 (2004),pp. 1206-1220
[52]	Wang, H., Avci, U., Nakashima, J. et al. Mutation of WRKY transcription factors initiates pith secondary wall formation and increases stem biomass in dicotyledonous plants Proc. Natl. Acad. Sci. U. S. A., 107 (2010),pp. 22338-22343
[53]	Wang, H., Boavida, L., Ron, M. et al. Plant Cell, 20 (2008),pp. 3300-3311
[54]	West, M., Harada, J.J. Embryogenesis in higher plants: an overview Plant Cell, 5 (1993),pp. 1361-1369
[55]	West, M., Yee, K.M., Danao, J. et al. Plant Cell, 6 (1994),pp. 1731-1745
[56]	Yang, H., Kaur, N., Kiriakopolos, S. et al. Planta, 224 (2006),pp. 1004-1014