Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice

Rongfang Xu; Yachun Yang; Ruiying Qin; Hao Li; Chunhong Qiu; Li Li; Pengcheng Wei; Jianbo Yang

doi:10.1016/j.jgg.2016.07.003

Volume 43 Issue 8

Aug. 2016

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Rapid improvement of grain weight via highly efficient CRISPR/Cas9-mediated multiplex genome editing in rice

doi: 10.1016/j.jgg.2016.07.003

Rongfang Xu^{1, 2, #},
Yachun Yang^{1, 2, #},
Ruiying Qin³,
Hao Li³,
Chunhong Qiu³,
Li Li^{4, 5},
Pengcheng Wei⁶,
Jianbo Yang^{7, 8}

1
Key Laboratory of Rice Genetic Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
2
School of Agronomy, Anhui Agricultural University, Hefei 230036, China
3
Key Laboratory of Rice Genetic Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
4
Key Laboratory of Rice Genetic Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
5
School of Agronomy, Anhui Agricultural University, Hefei 230036, China
6
Key Laboratory of Rice Genetic Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
7
Key Laboratory of Rice Genetic Breeding of Anhui Province, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230031, China
8
School of Agronomy, Anhui Agricultural University, Hefei 230036, China

More Information

Corresponding author: E-mail address: weipengcheng@gmail.com (Pengcheng Wei); E-mail address: yjianbo@263.net (Jianbo Yang)
Received Date: 2016-02-26
Accepted Date: 2016-07-20
Rev Recd Date: 2016-07-10

Available Online: 2016-07-29

Publish Date: 2016-08-20

Abstract

These authors contributed equally to this work.

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References(10)

References

[1]	Lei, Y., Lu, L., Liu, H.-Y. et al. CRISPR-P: a web tool for synthetic single-guide RNA design of CRISPR-system in plants Mol. Plant, 7 (2014),pp. 1494-1496
[2]	Ma, X., Zhang, Q., Zhu, Q. et al. A robust CRISPR/Cas9 system for convenient, high-efficiency multiplex genome editing in monocot and dicot plants Mol. Plant, 8 (2015),pp. 1274-1284
[3]	Shan, Q., Zhang, Y., Chen, K. et al. Plant Biotechnol. J., 13 (2015),pp. 791-800
[4]	Wang, C., Shen, L., Fu, Y. et al. A simple CRISPR/Cas9 system for multiplex genome editing in rice J. Genet. Genomics, 42 (2015),p. 703
[5]	Xie, K., Minkenberg, B., Yang, Y. Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system Proc. Natl. Acad. Sci. U. S. A., 112 (2015),pp. 3570-3575
[6]	Xing, H.-L., Dong, L., Wang, Z.-P. et al. A CRISPR/Cas9 toolkit for multiplex genome editing in plants BMC Plant Biol., 14 (2014),p. 327
[7]	Xu, R.-F., Li, H., Qin, R.-Y. et al. Generation of inheritable and “transgene clean” targeted genome-modified rice in later generations using the CRISPR/Cas9 system Sci. Rep., 5 (2015)
[8]	Zhang, H., Zhang, J., Wei, P. et al. The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation Plant Biotechnol. J., 12 (2014),pp. 797-807
[9]	Zhang, Z., Mao, Y., Ha, S. et al. Plant Cell Rep., 7 (2015),pp. 1519-1533
[10]	Zuo, J., Li, J. Molecular genetic dissection of quantitative trait loci regulating rice grain size Annu. Rev. Genet., 48 (2014),pp. 99-118

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