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Volume 44 Issue 2
Feb.  2017
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Article Navigation >  Journal of Genetics and Genomics  > 2017  >  44(2): 85-94

ISWI ATP-dependent remodeling of nucleoplasmic ω-speckles in the brain of Drosophila melanogaster

doi: 10.1016/j.jgg.2016.12.002
  • a 

    STEBICEF Department, University of Palermo, Palermo 90128, Italy

  • b 

    Genomix4Life Srl, University of Salerno, Baronissi Campus, Salerno 84081, Italy

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  • Corresponding author: E-mail address: mconorati@dti.telethon.it (Maria Cristina Onorati)
  • Received Date: 2016-07-29
  • Accepted Date: 2016-12-18
  • Rev Recd Date: 2016-11-21
    • Available Online: 2016-12-22
  • Publish Date: 2017-02-20
    Abstract
  • Heterogeneous nuclear ribonucleoproteins (hnRNPs) belong to the RNA-binding proteins family. They are involved in processing heterogeneous nuclear RNAs (hnRNAs) into mature mRNAs. These proteins participate in every step of mRNA cycle, such as mRNA export, localization, translation, stability and alternative splicing. At least 14 major hnRNPs, which have structural and functional homologues in mammals, are expressed inDrosophila melanogaster. Until now, six of these hnRNPs are known to be nucleus-localized and associated with the long non-coding RNA (lncRNA) heat shock responsive ω (hsrω) in the omega speckle compartments (ω-speckles). The chromatin remodeler ISWI is the catalytic subunit of several ATP-dependent chromatin-remodeling complexes, and it is an essential factor for organization of ω-speckles. Indeed, in ISWI null mutant, severe defects in ω-speckles structure are detectable. Here, we clarify the role of ISWI in the hnRNPs‒hsrω interaction. Moreover, we describe how ISWI by its remodeling activity, controls hsrω and hnRNPs engagement in ω-speckles. Finally, we demonstrate that the sequestration of hnRNPs in ω-speckles nuclear compartment is a fundamental event in gene expression control and represents a key step in the regulation of several pathways.
    • Current address: Turku Center for Biotechnology, University of Turku, Turku FIN-20520, Finland.
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    Periodical cited type(7)

    1. Singh, A.K.. Hsrω and Other lncRNAs in Neuronal Functions and Disorders in Drosophila. Life, 2023, 13(1): 17. doi:10.3390/life13010017
    2. Piccolo, L.L., Mochizuki, H., Nagai, Y. The lncRNA hsrω regulates arginine dimethylation of human FUS to cause its proteasomal degradation in Drosophila. Journal of Cell Science, 2019, 132(20): jcs236836. doi:10.1242/jcs.236836
    3. Muraoka, Y., Nakamura, A., Tanaka, R. et al. Genetic screening of the genes interacting with Drosophila FIG4 identified a novel link between CMT-causing gene and long noncoding RNAs. Experimental Neurology, 2018. doi:10.1016/j.expneurol.2018.08.009
    4. Lo Piccolo, L., Bonaccorso, R., Attardi, A. et al. Loss of ISWI function in Drosophila nuclear bodies drives cytoplasmic redistribution of Drosophila TDP-43. International Journal of Molecular Sciences, 2018, 19(4): 1082. doi:10.3390/ijms19041082
    5. Lo Piccolo, L.. Drosophila as a model to gain insight into the role of lncRNAs in neurological disorders. Advances in Experimental Medicine and Biology, 2018. doi:10.1007/978-981-13-0529-0_8
    6. Lo Piccolo, L., Jantrapirom, S., Nagai, Y. et al. FUS toxicity is rescued by the modulation of lncRNA hsrω expression in Drosophila melanogaster. Scientific Reports, 2017, 7(1): 15660. doi:10.1038/s41598-017-15944-y
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