Mapping quantitative trait loci using binned genotypes

Wen Yao; Guangwei Li; Yanru Cui; Yiming Yu; Qifa Zhang; Shizhong Xu

doi:10.1016/j.jgg.2019.06.005

Volume 46 Issue 7

Jul. 2019

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Article Navigation > Journal of Genetics and Genomics > 2019 > 46(7): 343-352

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Mapping quantitative trait loci using binned genotypes

doi: 10.1016/j.jgg.2019.06.005

Wen Yao^{a, c, #},
Guangwei Li^{a, #},
Yanru Cui^d,
Yiming Yu^a,
Qifa Zhang^a,
Shizhong Xu^b

a
National Key Laboratory of Crop Genetic Improvement and National Centre of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan, 430070, China
b
Department of Botany and Plant Sciences, University of California Riverside, Riverside, CA, 92521, USA
c
National Key Laboratory of Wheat and Maize Crop Science, College of Life Sciences, Henan Agricultural University, Zhengzhou, 450002, China
d
College of Agronomy, Hebei Agricultural University, Baoding, 071001, China

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Corresponding author: E-mail address: qifazh@mail.hzau.edu.cn (Qifa Zhang); E-mail address: shizhong.xu@ucr.edu (Shizhong Xu)
Received Date: 2018-12-31
Accepted Date: 2019-06-21
Rev Recd Date: 2019-06-16

Available Online: 2019-07-23

Publish Date: 2019-07-20

Abstract

Abstract

Precise mapping of quantitative trait loci (QTLs) is critical for assessing genetic effects and identifying candidate genes for quantitative traits. Interval and composite interval mappings have been the methods of choice for several decades, which have provided tools for identifying genomic regions harboring causal genes for quantitative traits. Historically, the concept was developed on the basis of sparse marker maps where genotypes of loci within intervals could not be observed. Currently, genomes of many organisms have been saturated with markers due to the new sequencing technologies. Genotyping by sequencing usually generates hundreds of thousands of single nucleotide polymorphisms (SNPs), which often include the causal polymorphisms. The concept of interval no longer exists, prompting the necessity of a norm change in QTL mapping technology to make use of the high-volume genomic data. Here we developed a statistical method and a software package to map QTLs by binning markers into haplotype blocks, called bins. The new method detects associations of bins with quantitative traits. It borrows the mixed model methodology with a polygenic control from genome-wide association studies (GWAS) and can handle all kinds of experimental populations under the linear mixed model (LMM) framework. We tested the method using both simulated data and data from populations of rice. The results showed that this method has higher power than the current methods. An R package named binQTL is available from GitHub.
- Genome-wide association studies,
- Linear mixed model,
- Polygene,
- Proximal contamination,
- QTL mapping

These authors contributed equally to this work.

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References(32)

References

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1.	Ouyang Y., Li X., Zhang Q. Understanding the genetic and molecular constitutions of heterosis for developing hybrid rice. Journal of Genetics and Genomics, 2022. doi:10.1016/j.jgg.2022.02.022
2.	Satrio R.D., Fendiyanto M.H., Supena E.D.J. et al. Genome-wide SNP discovery, linkage mapping, and analysis of QTL for morpho-physiological traits in rice during vegetative stage under drought stress. Physiology and Molecular Biology of Plants, 2021, 27(11): 2635-2650. doi:10.1007/s12298-021-01095-y

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