A single-cell atlas of mouse olfactory bulb chromatin accessibility

Yin Chen; Xiangning Ding; Shiyou Wang; Peiwen Ding; Zaoxu Xu; Jiankang Li; Mingyue Wang; Rong Xiang; Xiaoling Wang; Haoyu Wang; Qikai Feng; Jiaying Qiu; Feiyue Wang; Zhen Huang; Xingliang Zhang; Gen Tang; Shengping Tang

doi:10.1016/j.jgg.2021.02.007

Volume 48 Issue 2

Feb. 2021

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Article Navigation > Journal of Genetics and Genomics > 2021 > 48(2): 147-162

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A single-cell atlas of mouse olfactory bulb chromatin accessibility

doi: 10.1016/j.jgg.2021.02.007

Yin Chen^{a, b, #},
Xiangning Ding^{a, b, #},
Shiyou Wang^{a, b, #},
Peiwen Ding^{a, b},
Zaoxu Xu^{a, b},
Jiankang Li^a,
Mingyue Wang^{a, b},
Rong Xiang^{a, b},
Xiaoling Wang^{a, b},
Haoyu Wang^{a, b},
Qikai Feng^{a, b},
Jiaying Qiu^{a, b},
Feiyue Wang^{a, b, c},
Zhen Huang^d,
Xingliang Zhang^{e, f},
Gen Tang^e,
Shengping Tang^{e, g, h}

a
BGI Education Center, University of Chinese Academy of Sciences, Shenzhen 518083, China
b
BGI-Shenzhen, Shenzhen 518083, China
c
School of Biological Science & Medical Engineering, Southeast University, Nanjing 210096, China
d
Southern Center for Biomedical Research and Fujian Key Laboratory of Developmental and Neural Biology, College of Life Sciences, Fujian Normal University, Fuzhou, Fujian 350117, China
e
Shenzhen Children's Hospital, Shenzhen 518083, China
f
Department of Pediatrics, the Affiliated Hospital of Guangdong Medical University, Zhanjiang 524000, China
g
Zunyi Medical University, Zunyi, Guizhou 563099, China
h
China Medical University, Shenyang, Liaoning 110122, China

More Information

Corresponding author: E-mail address: xingliang_zhang@163.com (Xingliang Zhang); E-mail address: genine56@126.com (Gen Tang); E-mail address: tangshengping56@126.com (Shengping Tang)
Received Date: 2020-08-06
Accepted Date: 2021-02-01
Rev Recd Date: 2021-01-26

Available Online: 2021-03-17

Publish Date: 2021-02-20

Abstract

Abstract

Olfaction, the sense of smell, is a fundamental trait crucial to many species. The olfactory bulb (OB) plays pivotal roles in processing and transmitting odor information from the environment to the brain. The cellular heterogeneity of the mouse OB has been studied using single-cell RNA sequencing. However, the epigenetic landscape of the mOB remains mostly unexplored. Herein, we apply single-cell assay for transposase-accessible chromatin sequencing to profile the genome-wide chromatin accessibility of 9,549 single cells from the mOB. Based on single-cell epigenetic signatures, mOB cells are classified into 21 clusters corresponding to 11 cell types. We identify distinct sets of putative regulatory elements specific to each cell cluster from which putative target genes and enriched potential functions are inferred. In addition, the transcription factor motifs enriched in each cell cluster are determined to indicate the developmental fate of each cell lineage. Our study provides a valuable epigenetic data set for the mOB at single-cell resolution, and the results can enhance our understanding of regulatory circuits and the therapeutic capacity of the OB at the single-cell level.
- scATAC-seq,
- Mouse olfactory bulb,
- Chromatin accessibility,
- Transcription factor,
- Endogenous retroviral elements,
- Co-embedding analysis

These authors contributed equally to this work.

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References(106)

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