Genome sequence and transcriptome of <i>Sorbus pohuashanensis</i> provide insights into population evolution and leaf sunburn response

Dongxue Zhao; Yan Zhang; Yizeng Lu; Liqiang Fan; Zhibin Zhang; Jian Zheng; Mao Chai

doi:10.1016/j.jgg.2021.12.009

Volume 49 Issue 6

Jun. 2022

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Genome sequence and transcriptome of Sorbus pohuashanensis provide insights into population evolution and leaf sunburn response

doi: 10.1016/j.jgg.2021.12.009

a. School of Landscape Architecture, Beijing University of Agriculture, Beijing 102206, China;
b. Shandong Provincial Center of Forest and Grass Germplasm Resources, Jinan, Shandong 250102, China;
c. Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, Anyang, Henan 455000, China

Funds:

We thank the National Forest Genetic Resources Platform (NFGRP) for providing the S. pohuashanensis plant resources. This work was supported by the Fund of the National Natural Science Foundation of China (grant number 31770369) to J. Z. We also would like to thank Editage (www.editage.cn) for English language editing.

Received Date: 2021-09-23
Revised Date: 2021-12-09
Accepted Date: 2021-12-09
Publish Date: 2022-06-30

Abstract

Abstract

Sorbus pohuashanensis (Hance) Hedl. is a potential horticulture and medicinal plant, but its genomic and genetic backgrounds remain unknown. Here, we sequence and assemble the S.pohuashanensis reference genome using PacBio long reads. Based on the new reference genome, we resequence a core collection of 22 Sorbus spp. samples, which are divided into 2 groups (G1 and G2) based on phylogenetic and PCA analyses. These phylogenetic clusters are highly consistent with their classification based on leaf shape. Natural hybridization between the G1 and G2 groups is evidenced by a sample (R21) with a highly heterozygous genotype. Nucleotide diversity (π) analysis shows that G1 has a higher diversity than G2 and that G2 originated from G1. During the evolution process, the gene families involved in photosynthesis pathways expanded and the gene families involved in energy consumption contracted. RNA-seq data suggests that flavonoid biosynthesis and heat-shock protein (HSP)-heat-shock factor (HSF) pathways play important roles in protection against sunburn. This study provides new insights into the evolution of Sorbus spp. genomes. In addition, the genomic resources, and the identified genetic variations, especially those related to stress resistance, will help future efforts to produce and breed Sorbus spp.
- Sorbus pohuashanensis,
- Genome assembly,
- Population evolution,
- Sunburn

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