Generation of pigs with humanized type II collagen by precise human <i>COL2A1</i> gene knock-in

Ting Lan; Yuling Zheng; Yangyang Suo; Yuhui Wei; Hui Shi; Quanmei Yan; Zhenpeng Zhuang; Huangyao Chen; Quanjun Zhang; Nana Fan; Yu Zhao; Zhen Ouyang; Chengdan Lai; Zhaoming Liu; Jizeng Zhou; Chengcheng Tang; Nam-Hyung Kim; Qingjian Zou; Xiaomin Wang

doi:10.1016/j.jgg.2022.05.001

Volume 50 Issue 3

Mar. 2023

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Article Navigation > Journal of Genetics and Genomics > 2023 > 50(3): 212-215

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Generation of pigs with humanized type II collagen by precise human COL2A1 gene knock-in

doi: 10.1016/j.jgg.2022.05.001

1. Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Wuyi University, Jiangmen, Guangdong 529020, China;
2. University of Chinese Academy of Sciences, Beijing 100049, China;
3. CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China;
4. Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Wuyi University, Jiangmen, Guangdong 529020, China;
5. Joint School of Life Science, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou Medical University, Guangzhou, Guangdong 510530, China;
6. Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Wuyi University, Jiangmen, Guangdong 529020, China;
7. CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China;
8. The School of Biomedical and Pharmaceutical Sciences, Guangdong University of Technology, Guangzhou, Guangdong 510006, China;
9. Guangdong Provincial Key Laboratory of Large Animal Models for Biomedicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Wuyi University, Jiangmen, Guangdong 529020, China;
10. CAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, South China Institute for Stem Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong 510530, China;
11. School of Life Sciences, Westlake University, Hangzhou, Zhejiang 310024, China;
12. Institute of Biology, Westlake Institute for Advanced Study, Hangzhou, Zhejiang 310024, China

Funds:

This work was financially supported by National Key Research and Development Program of China (2017YFA0105103), the Strategic Priority Research Program of the Chinese Academy of Sciences (XDA16030503), Key Research & Development Program of Bioland Laboratory (Guangzhou Regenerative Medicine and Health Guangdong Laboratory) (2018GZR110104004), Science and Technology Planning Project of Guangdong Province, China (2020B1212060052, 2017A050501059), Science and Technology Program of Guangzhou, China (202007030003), Research Unit of Generation of Large Animal Disease Models, Chinese Academy of Medical Sciences (2019-I2M-5-025), the Science Foundation for Young Teachers of Wuyi University (2019TD05), Natural Science Foundation of Guangdong Province of China (2019A1515110283), Jiangmen Science and Technology Plan Project (2020JC01030).

Received Date: 2022-01-14
Accepted Date: 2022-05-03
Rev Recd Date: 2022-04-30
Publish Date: 2022-05-20

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References(14)

References

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