Genomic allele-specific base editing with imperfect gRNA

Xu-Xu Chen; Dong-Dong Zhao; Xue-Ting Hou; Ju Li; Shi-Ming Pu; Ji-Dong Fei; Si-Wei Li; Zu-Ping Zhou; Chang-Hao Bi; Xue-Li Zhang

doi:10.1016/j.jgg.2023.05.010

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Genomic allele-specific base editing with imperfect gRNA

doi: 10.1016/j.jgg.2023.05.010

Xu-Xu Chen^{1, 2, 3, 4},
Dong-Dong Zhao^{2, 3, 4},
Xue-Ting Hou^{1, 2, 3, 5},
Ju Li⁶,
Shi-Ming Pu^{1, 7},
Ji-Dong Fei^{2, 3, 4},
Si-Wei Li^{2, 3, 4},
Zu-Ping Zhou^{1, 7},
Chang-Hao Bi^{2, 3, 4},
Xue-Li Zhang^{2, 3, 4}

1
School of Life Sciences, Guangxi Normal University, Guilin, Guangxi 541006, China
2
Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
3
National Technology Innovation Center of Synthetic Biology, Tianjin 300308, China
4
Key Laboratory of Systems Microbial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China
5
Hubei College of Chinese Medicine, JingZhou, Hubei 434020, China
6
College of Life Science, Tianjin Normal University, Tianjin 300387, China
7
Guangxi Universities Key Laboratory of Stem Cell and Biopharmaceutical Technology, Guangxi Normal University, Guilin, Guangxi 541006, China

Funds: This research was financially supported by the National Key Research and Development Program of China (2018YFA0904900), the National Natural Science Foundation of China (32225031, 32171449, 81903776), the Tianjin Synthetic Biotechnology Innovation Capacity Improvement Project (TSBICIP-KJGG-017), the Tianjin Natural Science Foundation (20JCYBJC00310), and the Youth Innovation Promotion Association CAS (2022177).

Received Date: 2023-02-14
Revised Date: 2023-05-19
Accepted Date: 2023-05-22

Available Online: 2023-06-01

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References(17)

References

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