Rab1 and Syntaxin 17 regulate hematopoietic homeostasis through β-integrin trafficking in <i>Drosophila</i>

Fangzhou Luo; Luwei Sui; Ying Sun; Zhixian Lai; Chengcheng Zhang; Gaoqun Zhang; Bing Bi; Shichao Yu; Li Hua Jin

doi:10.1016/j.jgg.2024.11.001

Volume 52 Issue 1

Jan. 2025

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Article Navigation > Journal of Genetics and Genomics > 2025 > 52(1): 51-65

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Rab1 and Syntaxin 17 regulate hematopoietic homeostasis through β-integrin trafficking in Drosophila

doi: 10.1016/j.jgg.2024.11.001

a. College of Life Sciences, Northeast Forestry University, Harbin, Heilongjiang 150040, China;
b. Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany

Funds:

We thank Utpal Banerjee, Lucas Waltzer, Jiwon Shim, Jose´ Carlos Pastor-Pareja, Chao Tong, Erjun Ling and Rui Zhou for gifting the fly strains and Istvan Ando´, Martin Lowe, Ed Laufer, Yue Dong, Yangguang Hao, Michèle Crozatier and Developmental Studies Hybridoma Bank for providing the antibodies. We thank the Bloomington Drosophila Stock Center, Vienna Drosophila RNAi Center and TsingHua Fly Center for providing the fly stocks and Core Facility of Drosophila Resource and Technology, CEMCS, CAS for constructing transgenic flies. This work was supported by the National Natural Science Foundation of China (32170484 and 32300384), and the Fundamental Research Funds for the Central Universities (2572022DQ07 and 2572020AW04).

Received Date: 2024-07-19
Accepted Date: 2024-11-05
Rev Recd Date: 2024-11-01

Available Online: 2025-07-11

Publish Date: 2024-11-13

Abstract

Abstract

Hematopoiesis is crucial for organismal health, and Drosophila serves as an effective genetic model due to conserved regulatory mechanisms with vertebrates. In larvae, hematopoiesis primarily occurs in the lymph gland, which contains distinct zones, including the cortical zone, intermediate zone, medullary zone, and posterior signaling center (PSC). Rab1 is vital for membrane trafficking and maintaining the localization of cell adhesion molecules, yet its role in hematopoietic homeostasis is not fully understood. This study investigates the effects of Rab1 dysfunction on β-integrin trafficking within circulating hemocytes and lymph gland cells. Rab1 impairment disrupts the endosomal trafficking of β-integrin, leading to its abnormal localization on cell membranes, which promotes lamellocyte differentiation and alters progenitor dynamics in circulating hemocytes and lymph glands, respectively. We also show that the mislocalization of β-integrin is dependent on the adhesion protein DE-cadherin. The reduction of β-integrin at cell boundaries in PSC cells leads to fewer PSC cells and lamellocyte differentiation. Furthermore, Rab1 regulates the trafficking of β-integrin via the Q-SNARE protein Syntaxin 17 (Syx17). Our findings indicate that Rab1 and Syx17 regulate distinct trafficking pathways for β-integrin in different hematopoietic compartments and maintain hematopoietic homeostasis of Drosophila.
- Hematopoiesis,
- Lymph gland,
- Rab1,
- Syntaxin 17,
- β-integrin,
- Drosophila

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References(94)

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