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Volume 52 Issue 7
Jul.  2025
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Article Navigation >  Journal of Genetics and Genomics  > 2025  >  52(7): 927-941

The testis-specific gene 1700030J22Rik is essential for sperm flagellar function and male fertility in mice

doi: 10.1016/j.jgg.2024.12.010
  •  

    a. Institute of Reproductive Medicine, Medical School of Nantong University, Nantong, Jiangsu 226001, China;

  •  

    b. School of Basic Medical Science, Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetics of Ningxia Hui Autonomous Region, Ningxia Medical University, Yinchuan, Ningxia 750004, China;

  •  

    c. Department of Urology and Andrology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang 310016, China

Funds:

This work was supported by the National Key Research and Development Program of China (2024YFC2706800 to F.S.), the National Natural Science Foundation of China (U24A20657 F.S.), and the Key Research and Development Program of Zhejiang Province (2023C03035 to F.S.).

  • Received Date: 2024-09-30
  • Accepted Date: 2024-12-16
  • Rev Recd Date: 2024-12-13
    • Available Online: 2025-07-11
  • Publish Date: 2024-12-20
    Abstract
  • Spermiogenesis is an indispensable process occurring during the later stages of spermatogenesis. Despite multiple proteins being associated with spermiogenesis, the molecular mechanisms that control spermiogenesis remain poorly characterized. In this study, we show that 1700030J22Rik is exclusively expressed in testes of mice and investigate its roles in spermiogenesis using genetic and proteomic approaches. The deficiency in 1700030J22Rik in male mice results in severe subfertility, characterized by a substantial decrease in sperm concentration, motility, and abnormalities in the flagella. Furthermore, 1700030J22RIK interacts with the A-kinase-anchoring protein AKAP3, and 1700030J22Rik knockout decreases AKAP3 and AKAP4 protein levels. Additionally, the absence of 1700030J22RIK alters spermatozoal levels of the subunits of protein kinase A, leading to reduced protein phosphorylation and impaired sperm motility. This study reveals that 1700030J22Rik plays a crucial role in the organization of sperm morphology and function in mice.
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