Characteristic dysbiosis in gout and the impact of a uric acid-lowering treatment, febuxostat on the gut microbiota

Suxian Lin; Tao Zhang; Lingxiao Zhu; Kun Pang; Saisai Lu; Xin Liao; Senhong Ying; Lixia Zhu; Xin Xu; Jinyu Wu; Xiaobing Wang

doi:10.1016/j.jgg.2021.06.009

Volume 48 Issue 9

Sep. 2021

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Article Navigation > Journal of Genetics and Genomics > 2021 > 48(9): 781-791

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Characteristic dysbiosis in gout and the impact of a uric acid-lowering treatment, febuxostat on the gut microbiota

doi: 10.1016/j.jgg.2021.06.009

a. Rheumatology Department, Wenzhou People's Hospital, Wenzhou, Zhejiang 325000, China;
b. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, School of Life Sciences, Yunnan University, Kunming 650091, China;
c. Rheumatology Department, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, China;
d. Institute of Genomic Medicine, Wenzhou Medical University, Wenzhou, Zhejiang 325000, China;
e. Department of Rheumatology and Immunology, Shanghai Changzheng Hospital, Second Affiliated Hospital of Naval Medical University, Shanghai 200003, China

Funds:

Financial support is from the Project of Zhejiang Provincial Department of Education, China (Y202045471) and the Project of Wenzhou Science and Technology, China (NO. Y2020205).

Received Date: 2021-05-23
Revised Date: 2021-06-22
Accepted Date: 2021-06-24
Publish Date: 2021-07-09

Abstract

Abstract

Gut dysbiosis is suggested to play a critical role in the pathogenesis of gout. The aim of our study was to identify the characteristic dysbiosis of the gut microbiota in gout patients and the impact of a commonly used uric acid-lowering treatment, febuxostat on gut microbiota in gout. 16S ribosomal RNA sequencing and metagenomic shotgun sequencing was performed on fecal DNA isolated from 38 untreated gout patients, 38 gout patients treated with febuxostat, and 26 healthy controls. A restriction of gut microbiota biodiversity was detected in the untreated gout patients, and the alteration was partly restored by febuxostat. Biochemical metabolic indexes involved in liver and kidney metabolism were significantly associated with the gut microbiota composition in gout patients. Functional analysis revealed that the gut microbiome of gout patients had an enriched function on carbohydrate metabolism but a lower potential for purine metabolism, which was comparatively enhanced in the febuxostat treated gout patients. A classification microbial model obtained a high mean area under the curve up to 0.973. Therefore, gut dysbiosis characterizings gout could potentially serve as a noninvasive diagnostic tool for gout and may be a promising target of future preventive interventions.
- Gout,
- Gut microbiota,
- Febuxostat,
- Metabolism,
- Gene sequencing

These authors contribute equally to this work.

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References(49)

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