Two zinc-finger proteins control the initiation and elongation of long stalk trichomes in tomato

Ren Li; Xiaotian Wang; Shuaibin Zhang; Xin Liu; Zhen Zhou; Zhiqiang Liu; Ketao Wang; Yanbao Tian; Haijing Wang; Youjun Zhang; Xia Cui

doi:10.1016/j.jgg.2021.09.001

Volume 48 Issue 12

Dec. 2021

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Article Navigation > Journal of Genetics and Genomics > 2021 > 48(12): 1057-1069

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Two zinc-finger proteins control the initiation and elongation of long stalk trichomes in tomato

doi: 10.1016/j.jgg.2021.09.001

a. Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
b. Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
c. China State Key Laboratory of Subtropical Forest Cultivation, Zhejiang Agriculture and Forestry University, Hangzhou 311300, China;
d. State Key Laboratory of Plant Genomics and National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China

Funds:

This work was supported by the National Key Research and Development Program of China (2018YFD1000801), National Natural Science Foundation of China (32002050) and the Science Technology Innovation Program of the Chinese Academy of Agricultural Sciences.

Received Date: 2021-09-07
Revised Date: 2021-09-11
Accepted Date: 2021-09-13
Publish Date: 2021-12-20

Abstract

Abstract

Plant glandular trichomes are epidermal secretory structures that are important for plant resistance to pests. Although several regulatory genes have been characterized in trichome development, the molecular mechanisms conferring glandular trichome morphogenesis are unclear. We observed the differences in trichomes in cultivated tomato cv. ‘Moneymaker’ (MM) and the wild species Solanum pimpinellifolium PI365967 (PP), and used a recombinant inbred line (RIL) population to identify the genes that control trichome development in tomato. We found that the genomic variations in two genes, HAIR (H) and SPARSE HAIR (SH), contribute to the trichome differences between MM and PP. H and SH encode two paralogous C2H2 zinc-finger proteins that function redundantly in regulating trichome formation. Loss-of-function h/sh double mutants exhibited a significantly decreased number of Type I trichomes and complete loss of long stalk trichomes. Molecular and genetic analyses further indicate that H and SH act upstream of ZFP5. Overexpression of ZFP5 partially restored the trichome defects in NIL-h^PPsh^PP. Moreover, H and SH expression is induced by high temperatures, and their mutations inhibit the elongation of trichomes that reduce the plant repellent to whiteflies. Our findings confirm that H and SH are two vital transcription factors controlling initiation and elongation of Type I and III multicellular trichomes in tomato.
- H and SH,
- C2H2 zinc finger,
- Elongation,
- Long stalk trichome,
- ZFP5,
- Tomato

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References

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