OsbZIP27 coordinates with OsHUB1 and OsHUB2 to modulate drought tolerance in rice

Zuntao Xu; Yachun Yang; Fei Zhang; Hao Li; Hui Ma; Wenge Wu; Yong Ding

doi:10.1016/j.jgg.2024.11.016

Volume 52 Issue 2

Feb. 2025

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OsbZIP27 coordinates with OsHUB1 and OsHUB2 to modulate drought tolerance in rice

doi: 10.1016/j.jgg.2024.11.016

Zuntao Xu^a,b,c,d,
Yachun Yang^a,b,
Fei Zhang^c,d,
Hao Li^a,b,
Hui Ma^a,b,
Wenge Wu^a,b,e,
Yong Ding^c,d

a. Anhui Province Key Laboratory of Rice Genetics and Breeding, Rice Research Institute, Anhui Academy of Agricultural Sciences, Hefei, Anhui 230031, China;
b. Biological Breeding Laboratory of Anhui Province, Hefei, Anhui 230031, China;
c. Ministry of Education Key Laboratory for Membraneless Organelles and Cellular Dynamics, Hefei, Anhui 230027, China;
d. School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China;
e. The Grain Industry Research Institute of Anhui Agricultural University, Hefei, Anhui 230036, China

Received Date: 2024-08-12
Accepted Date: 2024-11-27
Rev Recd Date: 2024-11-26

Available Online: 2025-07-11

Publish Date: 2024-12-04

Abstract

Abstract

Histone H2B ubiquitination (H2Bub) is positively linked to transcriptional activation, but the genetic programs affected by H2Bub to enhance drought tolerance remain largely unknown. Here, we show that OsbZIP27 interacts directly with OsHUB1/2 to regulate drought tolerance in rice by binding to the promoters of OsHAK1 and OsGLN1 to achieve H2Bub and transcriptional activation. Consistently, mutations in OsbZIP27 reduce transcription of OsHAK1 and OsGLN1, resulting in increased sensitivity to drought stress. Moreover, loss of OsHUB1 and OsHUB2 function causes hypersensitivity to drought stress, whereas OsHUB2 overexpression enhances drought tolerance. Together, our results indicate that OsbZIP27 coordinates with OsHUB1/2 to enhance rice drought tolerance by increasing H2Bub and expression of OsHAK1 and OsGLN1.
- H2B monoubiquitination,
- Drought stress,
- bZIP transcription factor,
- Ring domain protein,
- Potassium transporter,
- Glucanase

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References

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